| 保肝颗粒中胡芦巴碱的含量测定 |
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| 引用本文： | 兰卫,赵保胜,景玉霞,徐暾海. 保肝颗粒中胡芦巴碱的含量测定[J]. 中国实验方剂学杂志, 2012, 18(11): 129-131 |
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| 作者姓名： | 兰卫 赵保胜 景玉霞 徐暾海 |
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| 作者单位： | 1. 新疆名医名方与特色方剂学自治区重点实验室,乌鲁木齐,830011
2. 北京中医药大学,北京,100029 |
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| 基金项目： | 新疆名医名方与特色方剂学重点实验室开放课题(20101015);自治区中医药实验教学示范中心科研课题(20100528) |
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| 摘 要： | 目的:建立高效液相法测定保肝颗粒中胡芦巴碱含量的方法.方法:以胡芦巴碱的含量为检验指标,AgilentZORBA×NH2柱色谱(4.6 mm×250 mm,5 μm),流动相水-甲醇-乙腈(20∶40∶40),流速1 mL· min-1,检测波长265 nm,进样量10μL,柱温40℃.结果:胡芦巴碱在进样量5～25 μL(r =0.999 9),线性关系良好,平均回收率为98.3％(n=9),RSD％1.63％.结论:该方法操作简单、快速、专属性强,其含量测定可为保肝颗粒的质量控制提供科学依据.
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| 关 键 词： | 保肝颗粒 胡芦巴碱 高效液相 含量测定 |
| 收稿时间： | 2011-11-10 |
Determination of Content of Trigonelline in Baogan Granulas |
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| LAN Wei,ZHAO Bao-sheng,JING Yu-xia and XU Tun-hai. Determination of Content of Trigonelline in Baogan Granulas[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(11): 129-131 |
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| Authors: | LAN Wei ZHAO Bao-sheng JING Yu-xia XU Tun-hai |
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| Abstract: | Objective:To establish a HPLC method for determination of trigonelline in Baogan granulas.Method: The content of trigonelline was determined by HPLC.The Agilent ZORBA×NH2 column(4.6 mm×250 mm,5 μm) was used and mobile phase was composed of water-methanol-acetonitrile(20∶40∶40).The flow rate of mobile phase was 1 mL·min-1 and detection wavelength was at 265 nm and the column temperature was maintained at 40 ℃.Result: The linear response range was from 5-25 μL(r=0.999 9).The recovery rate was 98.3%(n=9),RSD was 1.63%.Conclusion: HPLC method is simple,accurate and exclusive,which can provide the scientific basis for quality control of the Baogan granulas. |
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| Keywords: | Baogan granulas trigonelline HPLC content determination |
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