地塞米松对人肝癌细胞系FOXO1、PEPCK和PGC-1α表达的影响

目的研究地塞米松对FOXO1表达的影响以及FOXO1表达和磷酸烯醇丙酮酸羧激酶（PEPCK）及过氧化物酶体增生激活受体γ协同刺激因子1α（PGC-1α）表达之间的关系。方法采用RT-PCR技术和Western blot技术观察地塞米松刺激人肝癌细胞系（HepG2）后转录因子FOXO1、PCK1和PGC-1α的mRNA水平及蛋白水平的改变。结果500nmol／L地塞米松刺激HepGz细胞30min后，FOXO1 mRNA水平明显升高，约在1h达到最大值，刺激4h后，FoxO1蛋白水平也升高。地塞米松刺激的HepG2细胞FOXO1、PCK1和PGC-1α mRNA表达均在1h达到峰值，未发现三者在时间上的先后顺序。结论地塞米松能促进HepG2细胞中FOXO1、PEPCK和PGC-1α mRNA的同时表达。

Dexamethasone on expression of FOXO1、PEPCK and PGC-1α in hepatic cells(HepG2)

Objective To investigate the effects of dexamethasone on the expression of FOXO1 in hepatic cells （HepG2 cells） and relationship with expression of PCK1 and PGC-1α. Methods FOXO1 ,PEPCK（PCK1） and PGC-1α mRNA levels and protein levels in HepG2 cells cultured with dexamethasone were observed using RT-PCR technology and Western blot technology. Results Dexamethasone significantly increased the mRNA levels of FOXO1 in HepG2 cells, which reached the maximum by lb. Dexamethasone increased the protein level of FOXO1 paralleling with the mRNA levels. The time of reaching peaks of FOXO1, PCK1 and PGC-1α mRNA expression stimulated by dexamethasone was identical at lh, which meant that there was no sequence relationship among them. Conclusion Dexamethasone promotes the expression of FOXO1 in HepG2 cells. The changes in FOXO1 gene expression do not precede the changes in PCK1 and PGC-1α gene expression.