无饲养层条件原代培养小鼠胚胎干细胞的实验研究

目的:对无饲养层条件下原代分离培养小鼠胚胎干细胞(ESC)的可行性进行研究.方法:利用微滴培养技术,将小鼠胚泡分别置于无饲养层条件及人胚胎成纤维细胞(HEF)饲养层上进行培养.观察胚泡的发育行为,并对两种不同条件下ESC的克隆率进行研究.结果:在无饲养层及HEF饲养层条件下小鼠囊胚均可以正常发育,并可以原代培养出ESC...

Culture of Mural Embryonic Stem Cells Under Feeder-free Condition

Objective:To study the isolation and primary culture of mural embryonic stem cells(ESC)under feeder-free condition.Methods:With the microdrop culture technique,blastocysts were cultured under feeder-free condition and on HEF feeder layer respectively.Development of blastocysts and their behavior of praxiology were observed and the ESC isolation efficiency under feeder-free condition and on HEF feeder layer was studied simultaneously.Results:Blastocysts could normally develop under both feeder-free condition and on HEF feeder layer.Primary ESC could be isolated by the two culture systems.When compared with the group of HEF feeder layer,attachment rate of blastocysts after 48 h culture and ESC isolation efficiency were significantly lower under feeder-free condition(P<0.05).Conclusion:ESC can be primarily obtained under feeder-free condition.How to improve the ESC isolation efficiency needs further studies.