肝细胞癌合并门静脉癌栓组织中PDGF和survivin表达的意义

目的探讨血小板源性生长因子(PDGF)和凋亡抑制基因survivin在肝细胞癌合并门静脉癌栓组织中的表达及其与肝细胞癌细胞增殖之间的关系。方法应用原位杂交技术检测16例肝细胞癌合并门静脉癌栓组织中PDGFmRNA的表达,并与无门静脉癌栓者进行对照;应用免疫组化方法检测36例肝细胞癌合并门静脉癌栓组织中PDGF和survivin蛋白的表达及其相互关系,同时应用流式细胞仪检测肝细胞癌细胞的增殖情况及其分别与PDGF和survivin蛋白表达的关系。结果16例肝细胞癌合并门静脉癌栓组织中PDGFmRNA的表达量为0.1309±0.0146,显著高于无门静脉癌栓者的0.0684±0.0052(P<0.01);PDGF和survivin蛋白表达呈正相关关系(r=0.750,P<0.01);PDGF和survivin蛋白表达阳性组者的肝细胞癌细胞增殖显著高于表达阴性者(P<0.01)。结论肝细胞癌合并门静脉癌栓组织中存在PDGF和survivin基因的过度表达,且两者呈正相关关系;随着两基因表达的增强,肝细胞癌细胞增殖显著增高。

Significance of Expression of Platelet Derived Growth Factor and Survivin of Hepatocellular Carcinoma with Portal Vein Tumor Thrombosis

Objective To investigate the expressions of platelet derived growth factor (PDGF) and survivin in hepatocellular carcinoma (HCC) with portal vein tumor thrombosis (PVTT) and to evaluate the relationships among the expressions of PDGF, survivin and the proliferation of cancer cells. Methods The expression of PDGF mRNA in 16 cases in HCC with PVTT group was observed by in situ hybridization and the results were compared with that in HCC tissue group. The expressions of PDGF and survivin protein in 36 cases in HCC with PVTT group were detected with immunohistochemistry and it was found that there was a correlation between them. Flow cytometry (FCM) was used to measure the proliferation of cancer cells and it was also used to analyze the relations among PDGF, survivin and the proliferation of cancer cells. Results The expression level of PDGF mRNA in HCC with PVTT group was significantly higher than that in HCC tissue group (P<0.01). There was a positive correlation between the expressions of PDGF and survivin protein in HCC with PVTT (P< 0.01). The degree of proliferation of cancer cells in PDGF and survivin protein positive group was significantly higher than that in negative group (P< 0.01). Conclusion PDGF and survivin gene over-expressed in HCC with PVTT group and there is a positive correlation between the expressions of PDGF and survivin protein. The proliferation of cancer cells increases as the expressions of PDGF and survivin increase.