RP-HPLC法同时测定鸡冠花中槲皮素、木犀草素和山柰酚的含量

目的建立鸡冠花中槲皮素、木犀草素和山柰酚的含量测定方法。方法反相高效液相色谱法,色谱柱ZORBAXSB-C18(150mm×4.6mm,5μm);检测波长360nm;甲醇-0.2%磷酸(45∶55)为流动相;柱温30℃;流速1.0mL·min-1。结果槲皮素、木犀草素和山柰酚的回归曲线分别为:Y=1504.412X+9.9756,Y=1991.745X+8.6051,Y=567.591X+2.5397,它们分别在5.5×10-2～19.3×10-2mg·L-1,4.6×10-2～16.1×10-2mg·L-1,12.6×10-2～43.9×10-2mg·L-1范围内线性关系良好,相关系数r=0.99992～0.99998,加样回收率分别为94.68%,91.03%和103.08%,RSD分别为0.35%,1.01%和0.55%。样品分别含槲皮素、木犀草素和山柰酚0.176,0.262和0.105mg.g-1。结论方法简便可行,重复性好,数据及结果可靠。

Simultaneous determination of quercetin,luteolin and kaempferol in Celosia cristata L. By RP-HPLC

Objective To develop an RP-HPLC for determination of three flavonoids(quercetin,kaempferol and luteolin)in Celosia cristata L.Method The HPLC column was ZORBAX SB-C18(150 mm×4.6 mm,5 μm),temperature was 30 ℃;detection wavelength was 360 nm ;mobile phase was menthol-0.2%phosphonic acid(45∶55),flow rate was 1.0 mL·min-1.Results Quercetin,luteolin and kaempferol regression equations were Y=1 504.412X+9.975 6,Y=1 991.745X+8.6051 and Y=567.591X+2.539 7,respectively ;Linear ranges were 5.5×10-2～19.3×10-2 mg·L-1,4.6×10-2～16.1×10-2 mg·L-1 and 12.6×10-2～43.9×10-2 mg·L-1 respectively ;corelation coefficents were between 0.999 92 and 0.999 98;recoveries were 94.68%,91.03% and 103.08%,respectively ;RSD were 0.35%,1.01% and 0.55%,respectively.Sample contents of quercetin,luteolin and kaempferol were 0.176,0.262 and 0.105 mg·g-1,respectively.Conclusions This method was convenient and effective,and suitable for the determination of quercetin,kaempferol and luteolin in Celosia cristata L.