| 高效液相色谱法测定火麻仁油中Δ~9-四氢大麻酚的含量 |
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| 引用本文： | 张岗,郭江宁,毕开顺.高效液相色谱法测定火麻仁油中Δ~9-四氢大麻酚的含量[J].沈阳药科大学学报,2003(4). |
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| 作者姓名： | 张岗 郭江宁 毕开顺 |
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| 作者单位： | 沈阳药科大学药学院 辽宁沈阳110016
(张岗，郭江宁)，沈阳药科大学药学院 辽宁沈阳110016(毕开顺) |
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| 摘 要： | 目的建立火麻仁油中Δ9 四氢大麻酚的含量测定方法。方法采用Irregular H C18(2 5 0mm× 4 6mm ,10 μm)为色谱柱 ,以甲醇 乙腈 水 (10∶6 5∶2 5 ,V∶V∶V)为流动相的高效液相色谱法 ,流速 1 0mL·min-1,检测波长 2 2 0nm ,柱温室温。结果Δ9 四氢大麻酚在 1 2～9 6mg·L-1内呈良好的线性关系 (r =0 9998) ;样品平均加样回收率为 96 2 % ,RSD为 2 1%(n =9)。结论方法简便快速 ,适用于火麻仁油中Δ9 四氢大麻酚的定量分析
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| 关 键 词： | 火麻仁油 Δ9-四氢大麻酚 高效液相色谱法 |
Determination of Δ~9-tetrehydrocannabinol in the hempseed oil by HPLC |
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| ZHANG Gang,GUO Jiang ning,BI Kai shun.Determination of Δ~9-tetrehydrocannabinol in the hempseed oil by HPLC[J].Journal of Shenyang Pharmaceutical University,2003(4). |
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| Authors: | ZHANG Gang GUO Jiang ning BI Kai shun |
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| Abstract: | Objective To establish the analytical method for Δ 9 tetrahydrocannabinol in the hempseed oil.Methods The content of Δ 9 tetrahydrocannabinol was determined by HPLC.Irregular H C 18 ( 250 mm × 4 6 mm , 10 μm )was used.The mobile phase consisted of methanol,acetonitrile and water (10∶65∶25, V∶V∶V) .The flow rate was 1 0 mL·min -1 and the UV detector was set at 220 nm .The column temperature was room temperature.Results The calibration curve was good linear ( r =0 999 8 ) within the concentration range of 1 2～ 9 6 mg·L -1 for Δ 9 tetrahydrocannabinol.The average recovery and RSD were 96 2% and 2 1%( n =9) respectively.Conclusions The method is convenient,fast and suitable for the quality control of the Δ 9 tetrahydrocannabinol in the hempseed oil. |
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| Keywords: | hempseed oil Δ 9 tetrahydrocannabinol HPLC |
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