银强化滴金免疫法HCV—IgM检测临床价值 |
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引用本文: | 张铁汉,杨秀珍,刘琳,王伟,王伟娟.银强化滴金免疫法HCV—IgM检测临床价值[J].中国现代药物应用,2008,2(20):8-10. |
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作者姓名: | 张铁汉 杨秀珍 刘琳 王伟 王伟娟 |
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作者单位: | 张铁汉 (新乡医学院第三附属医院检验科,453003); 杨秀珍 (新乡医学院第三附属医院检验科,453003); 刘琳 (洛阳市中心医院); 王伟 (新乡医学院第三附属医院检验科,453003); 王伟娟 (新乡医学院第三附属医院检验科,453003); |
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摘 要: | 目的探讨丙型肝炎患者抗HCV—IgM检测的价值,方法对60名正常人、20例活动期丙型肝炎患者及10例稳定期丙型肝炎患者的血清进行抗HCV—IgM、HCV-RNA检测。结果两组抗HCV—IgM的阳性率比较差异有统计学意义(P〈0.001);慢性丙肝活动期患者的阳性率明显高于稳定期患者(P〈0.001);HCV—IgM抗体阳性率与HCV—RNA阳性率相比无差异统计学意义(P〉0.05)。结论血清抗HCV—IgM与肝细胞损害有关,可视为慢性丙型肝炎活动、HCV复制的标志。
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关 键 词: | 丙型肝炎病毒 IgM 免疫印迹法 免疫组织化学 |
Clinical value of HCV-lgM with silver enhanced dot immunogold fietration assay |
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Institution: | ZHANG Tie-han, YANG Xiu-zhen,LIU Lin, et al.( Department of Laboratory Medicine, the Third Affiliated Hospital of Xinxiang Medical College, Xinxiang 453003, China ) |
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Abstract: | Objective To evaluate clinical value of anti-HCV-IgM with silver enhanced dot immunogold fictration assay. Method The levels of anti-HCV-IgM and HCV-RNA in 60 healthy volunteers and in 20 patients with active hepatitis C and in 10 patients with stabilization period hepatitis C were determined with silver enhanced dot immunogold fietration assay(SEDIGFA). Results Anti HCV IgM was positive only in the patients with hepatitis C,and all negative in healthy donors;There were no cross reactions with IgM antibody to HAV and HBV;The false positive and negative caused by RF and specific IgG were removed; 30 patients with hepatitis C were assayed for anti-HCV IgM, the positive rate of 85% in the patients with active hepatitis C (ALT being abnormal) was significantly higher than that of 20% in the patients with stabilization period hepatitis C ( ALT being normal) ( P 〈 0. 001 ) ; the positive rate of anti HCV-IgM in the patients with hepatitis C was no significant difierence than HCV-RNA. Conclusion Anti-HCV IgM is a useful marker in diagnosis activity of chronic hepatitis C and guiding the antiviral therapy. |
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Keywords: | IgM |
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