| 固相萃取快速测定黑果枸杞果汁中酚酸类化合物 |
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| 引用本文： | 陈晨,文怀秀,赵晓辉,邵赟,梅丽娟.固相萃取快速测定黑果枸杞果汁中酚酸类化合物[J].中国中药杂志,2011,36(7):896-898. |
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| 作者姓名： | 陈晨 文怀秀 赵晓辉 邵赟 梅丽娟 |
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| 作者单位： | 1. 中国科学院,西北高原生物研究所,青海,西宁,810008;中国科学院,研究生院,北京,100049
2. 中国科学院,西北高原生物研究所,青海,西宁,810008 |
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| 基金项目： | 国家科技支撑计划项目(2007BAI45B00) |
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| 摘 要： | 目的:建立固相萃取-HPLC快速测定黑果枸杞果汁中6种酚酸(没食子酸、原儿茶素、儿茶素、绿原酸、咖啡酸、丁香酸).方法:经过固相萃取后的黑果枸杞果汁在色谱条件为:C18柱(4.6mm×250mm,5μm),流动相为甲醇-0.2%甲酸缓冲液,流速1 mL·min-1时进行梯度洗脱.结果:6种酚酸类化合物在一定的范围内有良好的线性关系,相关系数均为0.9999.平均回收率(n=5)分别为97.70%(RSD2.3%),99.64%(RSD1.8%),100.70%(RSD2.1%),99.98%(RSD2.6%),99.60%(RSD2.2%),99.04%(RSD2.4%).结论:通过固相萃取技术以及HPLC法测定6种酚酸类成分的含量,为黑果枸杞果汁的质量控制和资源开发提供了切实可行的方法.
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| 关 键 词： | 固相萃取 黑果枸杞果汁 酚酸 |
| 收稿时间： | 2010/8/30 0:00:00 |
Fast determination of phenolic acids in Lycium ruthenicum murr juice by solid phase extraction and HPLC |
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| CHEN Chen,WEN Huaixiu,ZHAO Xiaohui,TAO Yanduo and SHAO Yun.Fast determination of phenolic acids in Lycium ruthenicum murr juice by solid phase extraction and HPLC[J].China Journal of Chinese Materia Medica,2011,36(7):896-898. |
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| Authors: | CHEN Chen WEN Huaixiu ZHAO Xiaohui TAO Yanduo and SHAO Yun |
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| Institution: | Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining 810008, China;Graduate University of Chinese Academy of Sciences, Beijing 100049, China;Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining 810008, China;Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining 810008, China;Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining 810008, China;Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining 810008, China |
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| Abstract: | |
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| Keywords: | SPE Lycium ruthenicum phenolic components |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
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