叶酸对体外缺氧新生大鼠神经干细胞Hes5基因表达的影响

目的：探讨叶酸对体外缺氧新生大鼠神经干细胞肌s5mRNA、蛋白表达的影响。方法：分离培养24h新生SD大鼠噙神经干细胞：将细胞分为4组：正常对照组、缺氧模型组、缺氧叶酸添加组、缺氧叶酸缺乏组；于增殖第3天将除正常对照组外的其他3组细胞进行缺氧培养，6h后取出，继续培养至第6天收集细胞；用台盼蓝染色计‘数缺氧损伤后的各组细胞密度；RT—PCR方法检测Hes5mRNA表达；Westernblot方法检测Hes5蛋白表达。结果：缺氧叶酸添加组细胞密度高于其他各组．缺氧叶酸缺乏组最低，差异均有统计学意义（P〈0．05）；RT—PCR结果显示，缺氧叶酸添加组的Hes5mRNA表达量高于其余3组，缺氧叶酸缺乏组表达最少，各组间差异有统计学意义（P〈0．05）；Westernblot检测表明．缺氧叶酸添加组Hes5蛋白表达高于其他各组，缺氧叶酸缺乏组Hes5蛋白表达量最低，差异均有统计学意义（P〈0．05）。结论：体外神经干细胞缺氧损伤造模成功；叶酸对缺氧损伤的神经干细胞增殖有促进作用，可为预防和治疗脑缺血缺氧损伤提供依据。

Effects of folic acid on the expression of Hes5 of neural stem cells from neonatal rats in vitro after hypoxia damage

Objective:To explore the effect of folic acid (FA) on the mRNA and protein expression level of Hes5 of neural stem cells (NSCs) from neonatal rats in vitro after hypoxia damage.Methods:Cultured NSCs were randomly divided into four groups:normal control group,hypoxia model group,hypoxia FA-supplemented group and hypoxia FA deficiency group.On the third day of proliferation,the cells of three groups excepting the normal control group were cultured in hypoxia condition for 6 hours,then continued to culture unt...