不同胃液胃蛋白酶含量及活性的测定

目的：探讨红桂木凝集素用于胃液胃蛋白酶定量的可行性；证实胃蛋白酶在胃部疾病中的作用。方法：将红桂木凝集素（ＡＬＬ）标记上辣根过氧化物酶（ＨＲＰ），用夹心酶联法测定不同胃病患者胃液中胃蛋白酶的含量，并用改良安宋氏法测定其相应的酶活性。结果：正常组（１６例）、胃炎组（３２例）、十二指肠溃疡组（３２例）、胃溃疡组（２１例）及胃癌组（５例）中胃蛋白酶的含量及其活性均无显著性差异，但胃液ｐＨ值〈３的酶含量要

ASSAY OF PEPSIN CONTENT AND ACTIVITY IN VARIOUS GASTRIC JUICE

Objective:To prove the possibility of quantitating gastric juice pepsin by ALL,and to prove pepsin effects on stomach diseases Methods:The gastric juice pepsin contents and its activity were determined by ALL HRP sandwich assay and modification Anon's method respectively Result:There were no significant differences in both pepsin contents and its activity among the normal controls and patients with various gastric diseases including gastritis,duodenal ulcer,gastric ulcer and carcinoma of stomach But the pepsin contents of the gastric secretions with pH<3 were significantly higher than those with pH>4,the pepsin activities had no significant differences in the change of pH Conclusion:There is no closely relation between pepsin and stomach diseases Pepsin is no primary cause in producting stomach diseases