驱虫斑鸠菊提取物对小鼠免疫系统的影响

目的：探讨驱虫斑鸠菊提取物对小鼠免疫系统的影响.方法：驱虫斑鸠菊提取物高、中、低剂量组灌胃小鼠10 d,眼眶采血分离血清,用酶联免疫吸附法测定血清中IgA、IgG、IgM、C3、C4及IL-2的水平;解剖小鼠,测量小鼠胸腺和脾脏指数.以醋酸可的松作为阳性对照组,以生理盐水和溶媒作为空白照组和阴性对照.结果：①驱虫斑鸠菊提取物对小鼠免疫球蛋白IgA、IgG影响不大,对IgM、C3、C4有显著的降低作用;②驱虫斑鸠菊提取物对IL-2分泌有明显的抑制作用（P＜0.01）;③驱虫斑鸠菊提取物对正常小鼠胸腺指数、脾指数有显著降低作用（P＜0.01）.结论：驱虫斑鸠菊提取物对小鼠免疫系统具有明显抑制作用.

Effects of Extracts of Vernoniaanthelmintica Willd on Immune System of Normal Mice

Objective： To explore the effect of the Vemonia anthelmin -flea wiUd on the immune system of mice. Methods： VM extract high, medium and low dose mice lavage for 10 days, orbital blood serum separation, enzyme linked immunosorbent method for determina -tion of serum IgA, IgG, IgM, C3, C4 and the levels of IL - 2; Kill the mice and measure the thymus/spleen index. With cortisone acetate as the positive control group, with physiological saline and solvent as blank group and the negative control, results： 1） VM extract had little impact on the mice immune globulin IgA, IgG; but can decrease IgM, C3 and C4 level significantly ;（2） the VM extract can inhibitory the secretion of IL - 2 obviously （P 〈 0.01）;（3） the VM extract can reduced the of thymus/spleen index significantly on the normal miee（P 〈 0.01）. Conclusion： The VM extract has obvious inhibitory effect on the mice immune system.