基于^1H-NMR法研究3种非甾体抗炎药对KLA诱导的RAW264.7细胞膜磷脂的变化

目的应用1H-NMR法研究RAW264.7细胞不同炎症状态与细胞膜磷脂成分变化的相关性。方法RAW264.7细胞随机分为空白组、炎症组、双氯芬酸钠组、尼美舒利组、美洛昔康组,每组5皿细胞。采用100 ng·m L^-1KLA诱发RAW264.7细胞产生炎症,50μg·m L-1双氯芬酸钠、45μg·m L-1尼美舒利、30μg·m L-1美洛昔康分别干预建立给药组模型,TNF-α生成量变化验证模型,1H-NMR法采集各组细胞膜磷脂的氢谱,主成分分析法（PCA）分析细胞膜磷脂变化。结果细胞模型建立成功,1H-NMR图谱与PCA结合分析比较细胞膜磷脂,空白组、双氯芬酸钠组、尼美舒利组、美洛昔康组分别与炎症组距离较远,得分图分型较好,各组细胞膜磷脂差异均有统计学意义。结论 RAW264.7细胞膜磷脂在炎症前后发生了显著的变化,炎症的发生与磷脂的变化具有相关性。

Effect of three non-steroidal anti-inflammatory drugs on the change of phospholipids in KLA-stimulated RAW264.7 cells by ^1H-NMR

Objective To explore the relevance between inflammation and phospholipids in RAW264.7 cells by1H-NMR. Methods RAW264. 7 cells were divided into five groups,including the blank group,the inflammation group,the diclofenac sodium group,the nimesulide group and the meloxicam group. The inflammatory stimulation was established by 100 ng · m L^-1of KLA treatment. 50 μg · m L^-1diclofenac sodium,45 μg · m L^-1nimesulide and 30 μg · m L^-1meloxicam were added into the different groups,respectively. The levels of TNF-α in culture supernatant were measured. The changes of phospholipids were detected by1H-NMR spectra coupled with principal component analysis（ PCA）. Results A separation tendency of phospholipids was observed in the blank group and three treatment groups when compared with the inflammation group.Conclusion The phospholipids in RAW264.7 cells may be changed before and after inflammatory stimulation.