| 体外培养骨髓源性神经干细胞的细胞遗传学分析 |
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| 引用本文： | 金澎,徐如祥,姜晓丹,蔡颖谦,邹雨汐,杜谋选.体外培养骨髓源性神经干细胞的细胞遗传学分析[J].中华神经医学杂志,2005,4(1):16-19. |
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| 作者姓名： | 金澎 徐如祥 姜晓丹 蔡颖谦 邹雨汐 杜谋选 |
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| 作者单位： | 510282,广州,南方医科大学珠江医院神经外科 |
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| 基金项目： | 国家自然科学基金(3027047)，军队(01Z054)及广东省科技厅重大科技项目基金[No粤科基办(2000)25、粤财企(2001)367] |
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| 摘 要： | 目的评价体外培养的成人骨髓源性神经干细胞的遗传稳定性。方法制备骨髓源性神经干细胞的染色体标本，以常规染色和G显带进行染色体的核型分析。结果体外培养的成人骨髓源性神经干细胞的染色体数目为正常人类的46条染色体，未见非整倍体染色体像；G显带图像核型分析显示其为二倍体正常核型，染色体中未发现有缺失、倒位、易位、双着丝粒和环状染色体等结构异常。结论体外培养分化形成的骨髓源性神经干细胞保持了遗传学上的稳定性．为其进一步的临床应用提供了实验依据。
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| 关 键 词： | 骨髓源性神经干细胞 体外培养 G显带 染色体数目 临床应用 细胞遗传学分析 成人骨髓 着丝粒 核型分析 染色体标本 |
| 文章编号： | 1671-8925(2005)01-016-004 |
| 修稿时间： | 2004年8月18日 |
Cytogenetic analysis on bone marrow-derived neural stem cells cultured in vitro |
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| Jin Peng,XU Ru-xiang,JIANG Xiao-dan,CAI Ying-qian,Zou Yu-xi,Du Mou-xuan.Cytogenetic analysis on bone marrow-derived neural stem cells cultured in vitro[J].Chinese Journal of Neuromedicine,2005,4(1):16-19. |
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| Authors: | Jin Peng XU Ru-xiang JIANG Xiao-dan CAI Ying-qian Zou Yu-xi Du Mou-xuan |
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| Abstract: | Objective To evaluate the genetic stability of adult neural stem cells from bone marrow cultured in vitro. Methods Chromosome preparations of neural stem cells from bone marrow were made and analyzed on karyotypes using conventional staining and G-banding techniques. Results Chromosome number of neural stem cells from bone marrow was 46 as a normal human has and there was no aneuploid. The karyotypic analysis showed normal diploid, and no such chromosome structural abnormalities as deletion, inversion, translocation, dicentric and ring chromosome. Conclusion The bone marrow-derived neural stem cells cultured in vitro preserve cytogenetic stability, which provides experimental proof to bring this kind of cells into clinic application. |
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| Keywords: | Bone marrow cells Stem cells Cytogenetic analysis Karyotyping |
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