人CD7胞外区基因在大肠杆菌中的表达

目的 在大肠杆菌中表达人白细胞分化抗原CD7胞外区蛋白。方法 采用PCR技术调整CD7基因的阅读框架，使之与生物素化蛋白基因阅读框架一致，缺失了CD7cDNA基因的起始密码子并增加一个大肠杆菌偏性终止密码子，构建CD7胞外区cDNA和生物素化蛋白基因融合的原核表达质粒PinPointxa3-CD7。将重组质粒转入大肠杆菌DH5α表达，SDS-PAGE及Western blotting鉴定结果。结果 融合蛋白的分子量约为30000u,Western blotting结果表明，表达的蛋白质可被抗CD7的单克隆抗体识别。结论 为研制抗CD7基因工程抗体奠定了基础。

Expression of the extracellular domain of human CD7 in Escherichia coli

ObjectiveTo get the antigen of CD7.Methods The extracellular domain of human CD7 was cloned from a plasmid containing the full length of human CD7 cDNA and expressed in pinpoint xa3 prokaryotic system. Results Analysis with SDS PAGE and Western blotting showed that the expressed protein could bind to the anti CD7 mAb specifically and is about 30 000 u in molecular weight. Conclusion These results paved the way for preparing anti CD7 engineering antibody.