银屑病患者血清中IL-10与IL-21水平监测的临床应用

目的 通过检测银屑病患者血清中IL-10与IL-21表达水平,探讨其在银屑病病情进展程度及治疗效果中的意义。方法 按照赵辨主编的临床皮肤病学诊断标准,选择2014年3月～9月期间皮肤科就诊的门诊和住院寻常型银屑病患者65例,其中进行期25例(A组),稳定期20例(B组),恢复期20例(C组),并选择正常对照组25例(D组)。ELISA法检测各组血清中IL-10与IL-21表达水平,并对各组患者进行银屑病皮损面积和严重程度(PASI)评分。结果 A与B两组血清中IL-21表达水平分别为127.59±16.09 pg/ml和105.74±21.08 pg/ml,均明显高于D组85.46±14.25 pg/ml,差异有统计学意义(t值分别为5.174,4.863,P值均<0.01),而两组IL-10表达水平分别为10.64±3.23 pg/ml和12.27±2.18 pg/ml,均低于D组20.29±2.51 pg/ml,差异有统计学意义(t值分别为2.031,2.027,P值均<0.05)。C组血清中IL-21表达水平94.03±8.90 pg/ml高于D组,差异有统计学意义(t=2.033,P<0.05)。A组血清中IL-21表达水平高于C组,差异有统计学意义(t=2.352,P<0.05),IL-10表达水平低于C组19.69±1.54 pg/ml,差异有统计学意义(t=2.071,P<0.05)。银屑病患者血清中IL-21表达水平与PASI评分值正相关(r=0.508,P=0.027),而血清中IL-10表达水平与PASI评分为负相关(r=-0.413,P=0.039)。结论 银屑病的病情进展与患者血清中IL-21升高或IL-10降低有关,检测细胞因子IL-21和IL-10水平有助于判断其病情严重程度及疗效评估。

Clinical Application of IL-10 and IL-21 Levels Monitoring in Patients with Psoriasis

Objective To detect the level of IL-10 and IL-21 in serum of patients with psoriasis and explore its significance of judge the extent of serious condition and treatment of psoriasis.MethodsAccording to clinical dermatology edited by Zhao Bian diagnostic criteria,selected 65 cases of patients with psoriasis during the period from Marchto September in 2014 admitted to Department of Dermatology of out-patient andhospitalization.25 cases were progressive stage(group A),20 patients with stable stage(group B),20 patients were in recovery period(group C),and 25 casesof control group(group D).With ELISA method detect the expression level of IL-10 and IL-21 in serum of each group respectively.PASI score will take to allpsoriasis patients meanwhile.Results The expressionlevels of IL-21 in serum of A(127.59±16.09 pg/ml),B(105.74±21.08 pg/ml)two groups were significantly higher than that in D group(85.46±14.25 pg/ml),and the difference were statistically significant(t=5.174,4.863,both P<0.01),while the expression levels of IL-10 in A(10.64±3.23 pg/ml)and B(12.27±2.18 pg/ml)were both lower than D group(20.29±2.51 pg/ml),and the difference were statistically significant(t=2.031,2.027,both P<0.05).The expression of IL-21(94.03±8.90 pg/ml)in C group was higherthan that in D group,and the difference was statistically significant(t=2.033,P<0.05).The level of IL-21 in A group was higher than that in group C,and the difference was statistically significant(t=2.352,P<0.05),while the expression level of IL-10 was lower than that in C group(19.69±1.54 pg/ml),and the difference was statistically significant(t=2.071,P<0.05).The expression level of IL-21 in the serum of patients with psoriasis and PASI score was positively correlated(r=0.508,P=0.027),while the expressionlevel of IL-10 and PASI score was negatively correlated(r=-0.413,P=0.039).Conclusion The progression of psoriasis is related to the increasion of IL-21 or the decreasion of IL-10.The detection of IL-21and IL-10 was beneficial to judge the severity and efficacy evaluation of psoriasis.