Fibroblast growth factor receptor 2 gene amplification status and its clinicopathologic significance in gastric carcinoma |
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Authors: | Jung Eun-Jung Jung Eun-Ji Min Sun Young Kim Min A Kim Woo Ho |
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Affiliation: | a Department of Pathology, Seoul National University College of Medicine, Seoul 110-799, Republic of Koreab Department of Pathology, National Medical Center, Seoul 100-799, Republic of Koreac Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea |
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Abstract: | Fibroblast growth factor receptor 2 is a member of receptor tyrosine kinase family, and fibroblast growth factor receptor 2 gene amplification or missense mutation has been observed in various human cancers, including gastric carcinoma. Recent studies have shown that anti-fibroblast growth factor receptor 2 agents inhibit tumor progression in various human cancers, such as endometrial carcinoma and gastric carcinoma, which remains one of the most frequent causes of cancer-related death worldwide. We considered that knowledge of the status of fibroblast growth factor receptor 2 gene amplification in gastric carcinoma might aid in targeted cancer therapy. In this study, fibroblast growth factor receptor 2 amplification status was evaluated by fluorescence in situ hybridization in 313 surgically resected gastric carcinoma tissues, and the results were validated by quantitative real-time polymerase chain reaction. In addition, potential associations between clinicopathologic parameters and the presence of fibroblast growth factor receptor 2 amplification were investigated, and survival analysis was performed. Of the 313 cases, 14 (4.5%) showed fibroblast growth factor receptor 2 amplification by fluorescence in situ hybridization. Fibroblast growth factor receptor 2 amplification was found to be associated with a higher pT stage (P = .023), higher pN stage (P = .038), and distant metastasis (P = .009) and to be significantly associated with lower cancer-specific survival by univariate analysis (P = .012). Gastric carcinoma with fibroblast growth factor receptor 2 amplification was found to be associated with advanced disease and a poor prognosis. We believe that the determination of fibroblast growth factor receptor 2 amplification status could allow the identification of a subset of cancers sensitive to targeted fibroblast growth factor receptor 2 inhibitor-based therapy. |
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Keywords: | Stomach neoplasm Fibroblast growth factor receptor type II Fluorescence in situ hybridization Gene amplification Survival analysis |
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