三羟异黄酮对照射小鼠造血系统损伤的防护作用

目的　探讨三羟异黄酮 (Genistein)对辐射损伤小鼠造血系统的防护作用。方法　 6 0Gyγ射线全身照射BALB c雄性小鼠 ,分 4组 ,照射前 2 4h给予genistein灌胃 ,观察小鼠照射后 30d存活情况及不同时相点有关造血指标的变化 ,同时HE染色观察骨髓病理学改变。结果　Genistein处理组小鼠骨髓照后损伤较R组轻 ,外周血WBC、骨髓有核细胞数和CFU S下降幅度小且恢复较快(P <0 .0 5 ) ,同时genistein能明显延长辐射损伤小鼠的平均存活天数、提高小鼠 30d存活率 (P <0 0 5 ) ,以上结果均与已烯雌酚处理组相似 ,而且genistein对小鼠脾重系数的恢复也有一定的促进作用 (P <0 0 5 )。结论　Genistein能降低辐射对小鼠造血系统的损伤 ,具有一定的辐射保护作用。

Radioprotective effect of genistein on hematopoietic system of mice

Objective To study the radioprotective effect of genistein on mice hematopoietic system. Methods Adult male BALB/c mice were administered orally with genistein 24 h before exposure to a sublethal dose of 6^0 Gy gamma irradiation.The 30-day survival rate and some indices with relation to hematogenesis were examined after irradiation and pathologic changes of bone marrow were detected by hematotoxylin-eosin staining. Results Bone marrow was damaged slightly, and the numbers of peripheral blood WBC , bone marrow nucleated cells and CFU-S were all significantly higher and restored more rapidly in genistein-treated groups than those in the corresponding irradiated controls(P<0.05). Meanwhile,genistein could also increase the 30-days survival rate and the average survival days of dead mice(P<0.05). All results were similar to those in diethylstilbestrol-treated groups. In addition,genistein could promote recovery of spleen/body weight ratio (P<0.05). Conclusion These observations suggest that genistein has the capability of reducing the damage of hematopoietic system in irradiated mice and has significant protective effect on mice against radiation injury.;