大鼠深低温停循环复苏模型的建立

目的建立大鼠深低温停循环复苏模型。方法采用体外插管法建立大鼠闭胸式深低温停循环复苏模型,对其脑组织的病理形态学改变、超微结构的变化和动脉血气等各项指标进行检测。结果用大鼠建立深低温停循环复苏模型,呼吸的自主恢复率为80%,心跳恢复率为100%,同时对脑组织进行病理学观察,未见明显异常改变。结论大鼠深低温停循环复苏模型的建立,对研究脑组织低温状态下的病理生理学变化和临床应用低温进行脑保护提供了基本条件。

Establishment of animal model of deep hypothermia circulatory arrest in rats

Objective To establish the animal model of deep hypothermia circulatory arrest(DHCA)in rats. Methods We firstly established the animal model of closed chest DHCA in rats. Following the brain was given low flowing interval grade perfusion with cold cerebral protective fluid. The physiological signs of blood gas, the changes of pathology and ultra-structure were measured. Results The results suggested that the time limit was prolonged by the grading perfusion, and the period of cerebral hypoxia and ischemia were reduced at the same time. The recovery rates of respiration were 80% and those of the heart beat were 100%. There were no significant changes of the cerebral histological morphology. Conclusion A feasible model of DHCA in rats in which neurologic injury can be demonstrated, we believe that there are compelling reasons to use this model in the further study of both DHCA associated pathophysiology(both neurologic and other)and potential organ-protective strategies.