再生障碍性贫血患儿骨髓祖细胞培养的临床意义

目的探讨再生障碍性贫血(AA)患儿红细胞集落生成单位(CFU-E)、爆式红细胞集落生成单位(BFU-E)、粒细胞-单核细胞集落生成单位(CFU-GM)及巨核细胞集落生成单位(CFU-Meg)骨髓祖细胞培养情况,了解骨髓祖细胞在AA发病中的作用及意义。方法抽取28例AA患儿骨髓3～4mL,以淋巴细胞分离液提取单个核细胞,用磷酸盐缓冲液(PBS)洗涤2次,调节单个核细胞水平为106/mL,取0.3mL分别接种于红系、粒-单核系、巨核系培养基分别作4类祖细胞培养,在7、14d测定其集落数。结果28例AA中,4类集落数均值与对照组比较,差异有显著意义(P<0.01或P<0.001)。集落数随病情加重而逐步下降。4类集落数低于对照组患儿下限所占百分比分别为BFU-E35.71%、CFU-E85.71%、CFU-GM75.00%、CFU-Meg89.29%。治愈及进步者4类集落数接近对照组水平,治疗无效及进展者与治疗前比较无变化。结论动态监测AA患儿骨髓4类祖细胞集落数变化对辅助诊断、观察疗效及预后判断均有指导意义。

Clinical Significance and Culture of Hematopoietic Progenitor Cells in Children with Aplastic Anemia

Objective To study hematopoietic progenitor cells(HPC) proliferation though detecting blast-forming-unit of erythyrocyte(BFU-E),colony forming-unit of erythrocyte(CFU-E),colony-forming-unitofgranulocyte-monocyte(CFU-GM)and colony-forming-unit of megakayocyte(CFU-Meg)by HPC culture of bone marrow(BM) from children with aplastic anemia(AA).Methods Taking BM liquid 3-4 mL of children with AA,to detect clone formation unit(CFU) of BFU-E,CFU-E,CFU-GM and CFU-Meg at 7 d,14 d after cellular culture from 28 children with AA.Results The mean values of 4 different colonies in 28 children were significantly lower than those of the control BM.The mean decrease of these colonies was positively correlated with the severity of the types of diseases.Among 28 cases,the CFU counts of BFU-E(35.71%),CFU-E(85.71%),CFU-GM(75.00%),and CFU-Meg(89.29%) were lower than those of the control.Smilar to normal controls in the patients of cure.Conclusions A portion of children with AA have lower quantities of BFU-E,CFU-E,CFU-GM and CFU-Meg,and the quantitive decrease in these colonies is positively correlated with severity of the disease.Testing the changes of BFU-E,CFU-E,CFU-GM and CFU-Meg by HPC culture have signficanc to the assistant diagnosis,observeing effect and judging prognosis.