Evaluation of the anticocaine monoclonal antibody GNC92H2 as an immunotherapy for cocaine overdose |
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Authors: | Carrera M Rocío A Trigo José Manuel Wirsching Peter Roberts Amanda J Janda Kim D |
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Affiliation: | Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road (BCC 582), La Jolla, CA 92037, United States. rcarrera@scripps.edu |
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Abstract: | The illicit use of cocaine continues in epidemic proportions and treatment for cocaine overdose remains elusive. Current protein-based technology offers a new therapeutic venue by which antibodies bind the drug in the blood stream, inactivating its toxic effects. The therapeutic potential of the anticocaine antibody GNC92H2 was examined using a model of cocaine overdose. Swiss albino mice prepared with intrajugular catheters were tested in photocell cages after administration of 93 mg/kg (LD50) of cocaine and GNC92H2 infusions ranging from 30 to 190 mg/kg. GNC92H2 was delivered 30 min before, concomitantly or 3 min after cocaine treatment. Significant blockade of cocaine toxicity was observed with the higher dose of GNC92H2 (190 mg/kg), where premorbid behaviors were reduced up to 40%, seizures up to 77% and death by 72%. Importantly, GNC92H2 prevented death even post-cocaine injection. The results support the important potential of GNC92H2 as a therapeutic tool against cocaine overdose. |
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