Notochordal cells stimulate migration of cartilage end plate chondrocytes of the intervertebral disc in in vitro cell migration assays |
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| Authors: | Ki-Won Kim Kee-Yong Ha Jun-Seok Lee Suk-Woo Nam Young-Kyun Woo Tae-Hong Lim Howard S. An |
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| Affiliation: | 1. Krembil Research Institute, Toronto Western Hospital, 60 Leonard Ave, Toronto, Ontario M5T 2S8, Canada;2. Spine Surgery, University Hospital Basel, Spitalstr. 21, CH-4031 Basel, Switzerland;3. Division of Neurosurgery and Spine Program, University of Toronto, Toronto Western Hospital, 399 Bathurst St, Toronto, Ontario M5T 2S8, Canada;4. Division of Orthopaedic Surgery, University of Toronto, Toronto Western Hospital, 399 Bathurst St, Toronto, Ontario M5T 2S8, Canada;5. Division of Research, Canadian Memorial Chiropractic College, Toronto, Ontario M2H 3J1, Canada;1. School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China;2. Centre for Genomic Sciences, The University of Hong Kong, Pokfulam, Hong Kong SAR, China;3. The University of Hong Kong - Shenzhen Institute of Research and Innovation (HKU-SIRI), Hi-Tech Industrial Park, Nanshan, Shenzhen, China;4. Department of Orthopaedic Surgery, Washington University, St. Louis, MO 63110, USA;5. Department of Biology, Loyola University of Chicago, IL 60660, USA;6. Department of Genetics, Washington University, St. Louis, MO 63110, USA |
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| Abstract: | Background contextIt was recently demonstrated that the postnatal transition from a notochordal to a fibrocartilaginous nucleus pulposus (NP) is accomplished exogenously by chondrocytes migrating from hyaline cartilage end plates (CEs) into the ectopic notochordal NP region. Although our previous in vivo studies showed evidences for the migration of CE chondrocyte from hyaline CEs into the notochordal NP, it is unknown whether CE chondrocytes of the intervertebral disc (IVD) really have a motile property. In addition, the effect of notochordal cells on this property has not been elucidated.PurposeThe purpose of this in vitro study was to demonstrate whether CE chondrocytes of the IVD are capable of migration, and whether there is any biological link between notochordal cells and CE chondrocytes that may regulate the CE chondrocyte migration.Study design/settingIn vitro cell migration assays were performed using rat IVDs.MethodsNotochordal cells and chondrocytes were obtained from the NP and CE tissues, respectively, and were cultured separately. The different numbers of notochordal cells and the supernatant (conditioned medium) that contained soluble factors produced by notochordal cells were used to demonstrate their effects on the migration of CE chondrocytes. Bovine serum albumin (BSA) and lysophosphatidic acid (LPA) were used as negative and positive controls, respectively.ResultsCompared with BSA, LPA, notochordal cells (N=4×, 2×, 1×, and 0.5×105), and its conditioned media (unconcentrated and fivefold concentrated) significantly increased migration of CE chondrocytes (p<.05 in all comparisons). Particularly, notochordal cells and its conditioned media increased migration in a number- and concentration-dependent manner, respectively.ConclusionsThis study demonstrates that CE chondrocytes of the IVD are capable of migration and that soluble factors produced by notochordal cells stimulate the migration. These results provide a plausible explanation to the question of why CE chondrocytes of the IVD migrate into the ectopic NP region during the natural transition from the notochordal to fibrocartilaginous NP. |
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