PBMC对B7-2基因修饰的肝癌细胞Bel-7402体外杀伤作用的研究

目的研究B7-2基因修饰肿瘤后对T细胞活化的影响,探索B7-2在肿瘤免疫治疗中的作用。方法构建B7-2基因的表达载体,转染Bel-7402细胞,与外周血单核细胞（PBMC）共孵育,利用流式细胞术（FCM）检测Bel-7402细胞的凋亡。结果 B7-2基因修饰的Bel-7402细胞和PBMC细胞共培养4 h后,凋亡率为12.7%,与对照组（7.5%）比较,细胞凋亡率显著增加（P〈0.01）。结论 B7-2基因修饰能够增强肿瘤细胞对淋巴细胞的活化作用,为基于B7-2的抗肿瘤治疗奠定了基础。

PBMC killing-effect on the B7-2 gene-modified Bel-7402 hepatoma cells in vitro

Objective To study the influence of B7-2 gene modified tumor cells on T cells activation,and the role of B7-2 in tumor immunotherapy,and to explore new ideas on designing the malignant tumor comprehensive treatment based on B7-2. Methods Recombinant B7-2 gene expression vector was constructed,and Bel-7402 cells were tranfected,and then co-cultured with peripheral blood mononuclear cells（PBMC）,and Bel-7402 cells apoptosis were detected subsequently by flow cytometry（FCM）.Results Bel-7402 cells modified by B7-2 gene were co-cultured with PBMC for 4 h,and the apoptosis rate detected by flow cytometry was 12.7%,which was significantly increased compared with that in the control group（7.5%）（P0.01）.Conclusion B7-2 gene-modified tumor cells could enhance lymphocytic activation,which suggesting the feasibility of antitumor treatment based on B7-2.