RP-HPLC法测定陆英颗粒与陆英糖浆中乌索酸与齐墩果酸含量

目的测定陆英颗粒和陆英糖浆中乌索酸与齐墩果酸的含量。方法用RP HPLC法。色谱柱为Kromasil C18柱(250 mm×4.6 mm,5μm),流动相为甲醇水(体积比为87∶13),检测波长为210 nm,流速为1.0 mL.min-1,柱温为35℃,进样量为20μL。结果在该色谱条件下,乌索酸质量浓度在5.4~86.4 mg.L-1内,质量浓度与峰面积具有良好的线性关系,回归方程为A=9.58×103ρ-2.769×104,r=0.999 0;齐墩果酸质量浓度在5.2~83.2 mg.L-1内,质量浓度与峰面积具有良好的线性关系,回归方程为A=7.604×103ρ-2.253×104,r=0.999 0。陆英颗粒中乌索酸与齐墩果酸加样回收率分别为101.0%(RSD=1.9%)、99.6%(RSD=1.3%);陆英糖浆剂中乌索酸与齐墩果酸加样回收率分别为99.9%(RSD=1.4%)、100.3%(RSD=1.1%)。结论该法为陆英颗粒及陆英糖浆的质量控制提供了依据。

Determination of contents of ursolicacid and oleanolic acid in Syrupus Sambuci and Granulate Sambuci by RP-HPL

Objective To establish a method for the determination of the contents of ursolic acid and oleanolic acid in Syrupus Sambuci and Granulate Sambuci(traditional Chinese medicines).Methods RP-HPLC with Kromasil C_(18) column(250 mm×4.6 mm,5 μm) was used.The mobile phase was methanol-water(V∶V=87∶13),detection wavelengh was set at 210 nm.The flow rate was 1.0 mL·min~(-1),the column temperature was 35 ℃ and injection volumn was 20 μL.Results The ursolic acid calibration curve was linear at the range of 5.4-86.4 mg·L~(-1) and the regression equation was A=9.58×10~3ρ-2.769×10~4,r=0.999 0;The oleanolic acid calibration curve was linear at the range of 5.2-83.2 mg·L~(-1) and the regression equation was A=7.604×10~3ρ-2.253×10~4,r=0.999 0.The mean recoveries were 101.0%(RSD=1.9%),99.6%(RSD=1.3%),99.9%((RSD=)1.4%),and 100.3%(RSD=1.1%)for ursolic acid,oleanolic acid in granule,ursolic acid and oleanolic acid in syrup,respectively.Conclusions The method is simple,accurate and reproducible.It can be used in the quality control of Syrupus Sambuci and Granulate Sambuci.