Ribosomal control of translational fidelity in Podospora anserina: A suppressor and an antisuppressor affecting the paromomycin-induced misreading in vitro |
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Authors: | E. Coppin-Raynal |
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Affiliation: | (1) Laboratoire de Génétique de l'Université Paris-Sud, Centre d'Orsay (laboratoire associé au CNRS No 86), Bâtiment 400, F 91405 Orsay, France |
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Abstract: | Summary The hypersensitivity to paromomycin dis played by the su1–60 suppressor, which was thought to be a ribosomal ambiguity mutation, was used to isolate a new antisuppressor, AS71. This antisuppressor is not specific for ribosomal suppressors, since it is able to act on putative tRNA-type suppressors. Compared to the wild-type, the AS7-1 mutation produces an increased resistance to paromomycin. In addition, it was found to stop the sporulation process. In the polyuridylic acid-dependant assay, the paromomycin induced misreading was shown to be higher on su1–60 ribosomes and lower on ribosomes from AS7–1 mutant and AS7S-1-su1–60 double mutant than on wild-type ribosomes. These data confirm that su1–60 and AS7-1 mutants are ribosomal mutants involved in the control of translational fidelity. |
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Keywords: | Fungi Translational fidelity Ribosomal mutants Paromomycin |
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