Weight gain induced by an isocaloric pair-fed high fat diet: A nutriepigenetic study on FASN and NDUFB6 gene promoters |
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| Authors: | Almudena Lomba J. Alfredo Martínez Diego F. García-Díaz Laura Paternain Amelia Marti Javier Campión Fermín I. Milagro |
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| Affiliation: | 1. Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 16419, Korea;2. Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Wanju-Gun, Jeollabuk-do 55365, Korea;3. College of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Korea;4. Graduate School of East–West Medical Science, Kyung Hee University, Yongin 17104, Korea;1. Department of Nutrition, Food Sciences and Physiology, University of Navarra, Irunlarrea 1, 31008 Pamplona, Navarra, Spain;2. CIBERobn, Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid, Spain;1. Department of Biochemistry and Molecular Biology, Binzhou Medical College, Yantai, Shandong Province 264003, People''s Republic of China;2. Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People''s Republic of China;3. Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, Guangdong Province 523808, People''s Republic of China;4. Department of Biochemistry and Molecular Biology, Guizhou Medical University, Guiyang, Guizhou Province 550004, People''s Republic of China;5. Department of Medicine, College of Physicians and Surgeons, Columbia University New York, New York, NY 10027, USA |
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| Abstract: | Experimental studies have demonstrated that dietary macronutrient distribution plays an important role in insulin regulation, a risk factor associated to obesity, diabetes and other metabolic disorders. To assess whether the macronutrient composition of the diet could be related to obesity onset by affecting the epigenetic regulation of gene expression, we investigated in rats the metabolic effects of two pair-fed isocaloric diets: control (rich in carbohydrates) and high fat diet (rich in fat; HFD). Compared to controls, HFD induced higher weight gain and adiposity and impaired glucose tolerance, which was accompanied by a slight increase in adiponectin levels and liver steatosis. Epididymal adipose tissue expression of the fatty acid synthase (FASN) gene and NADH dehydrogenase (ubiquinone) 1β-subcomplex 6 (NDUFB6) were significantly reduced in HFD group. These variations in mRNA levels were accompanied by changes in the methylation patterns of several CpG islands located in the promoter region of these genes. However, no correlations were found between gene expression and the methylation status. These results suggest that high fat intake produces overweighted rats independently of total energy intake. These diets could also induce some epigenetic changes in the promoters of key genes that could influence gene expression and may be behind metabolic alterations. |
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