水通道蛋白8在人脑胶质瘤组织的表达及临床意义

目的:探讨水通道蛋白8(AQP8)在人脑胶质瘤中的表达及其与预后的关系。方法:选取河南省人民医院2017年7月至2019年7月35例人脑胶质瘤组织,通过实时荧光定量聚合酶链反应(PCR)技术检测AQP8的表达,再通过短发卡RNA(shRNA)技术下调AQP8基因,通过细胞增殖测定和Transwell侵袭/转移实验研究其...

Expression of aquaporin 8 in human glioma and clinical significance

Objective To investigate the expression of aquaporin 8(AQP8)in human glioma and its relationship with prognosis.Methods The expression of AQP8 was detected by real-time quantitative polymerase chain reaction(PCR)in 35 cases of human glioma tissues,and then AQP8 gene was down-regulated by short hairpin RNA(shRNA)technology.The effects of AQP8 on the proliferation and invasion/migration of u373 and T98G cells were studied by cell proliferation assay and Transwell invasion/metastasis experiment.T test or analysis of variance was used to compare the differences between groups.Results AQP8 expression level was up-regulated in glioma(1.52±0.36)as compared with normal brain tissue(t=7.969,P<0.01).After down-regulating AQP8,the relative proliferation rate of u373 and T98G cells was decreased at 72 h as compared with normal brain tissue(0.68±0.27,0.64±0.30,t=11.043,1.110,P<0.01).The expression of cyclins in u373 and T98G cells CCNB1(relative gray value,0.88±0.17,0.64±0.15,t=6.263,P<0.01),CCNB2(relative gray value,0.18±0.05,0.11±0.06,t=5.302,P<0.01),cyclin dependent kinase 1(CDK1,relative gray value,0.68±0.14,0.41±0.12,t=8.663,P<0.01),KIF4A(relative gray value,0.47±0.19,0.19±0.06,t=8.314,P<0.01)and FEN1(relative gray value,0.31±0.06,0.26±0.05,t=3.787,P<0.01)was significantly decreased.In addition,the invasion/migration ability of u373 and T98G cells was significantly decreased in the intervention group.Conclusion Down-regulation of AQP8 can inhibit the proliferation,invasion and migration of glioma cells.