微小RNA-146a靶向Nm23-H1对胃印戒细胞癌侵袭和转移的影响

目的:探讨微小RNA(miR)-146a靶向Nm23-H1对胃印戒细胞癌侵袭和转移的影响及其机制。方法:选取2018年12月至2020年12月漯河市中心医院与河南省人民医院收治的49例胃低分化印戒细胞癌和对应癌旁组织作为研究对象。采用miRNA微阵列分析分析胃癌组织和癌旁组织差异表达miRNA；采用荧光定量聚合酶链反应...

Effect of microRNA-146a targeting Nm23-H1 on invasion and metastasis of gastric signet-ring cell carcinoma

Objective To investigate the effect of microRNA(miR)-146a targeting nm23-H1 on invasion and metastasis of gastric signet-ring cell carcinoma and its molecular mechanism.Methods Totally 49 cases of poorly differentiated signet ring cell carcinoma of the stomach and corresponding adjacent tissues from December 2019 to December 2020 in Luohe Central Hospital and Henan Provincial People′s Hospital were selected as the research objects.miRNA microarray analysis was used to analyze the differentially expressed miRNAs in gastric cancer tissues and adjacent tissues.The differentially expressed miRNAs in gastric cancer tissues and adjacent tissues were verified by fluorescent quantitative polymerase chain reaction.Human gastric signet ring cell carcinoma cell line HSC-39 was infected with miRNA control and miR-146a inhibitor lentivirus to obtain stable cell line,serving as the control group and the experimental group,respectively.The invasion ability of the two groups was analyzed by Transwell.The metastasis ability of the two groups was analyzed by in vivo tumor inhibition experiment.The target gene of miR-146a was analyzed by bioinformatics and double luciferase reporter.The expression levels of target proteins were analyzed by Western blotting.T-test was used for data analysis,with P<0.05 as the difference.Results There were 84 miRNAs differentially expressed in tumor tissues and adjacent tissues,of which miR-146a was the most differentially expressed miRNA.The expression level of miR-146a in signet ring cell carcinoma of stomach(2.96±0.31)was significantly higher than that in adjacent tissues(1.29±0.23,t=3.104,P<0.05).As compared with the control group(1.02±0.19),the expression level of miR-146a in the experimental group(0.33±0.15)was significantly decreased(t=2.864,P<0.05).As compared with the control group(81.45±8.09)cells],the number of invasive cells in the experimental group(32.12±4.14)cells]decreased significantly(t=3.173,P<0.05).As compared with the control group(13.50±3.09)cells],the number of lymph node metastases in the experimental group(6.01±1.89)]decreased significantly(t=2.185,P<0.05).Nm23-H1 is a target protein of miR-146a.As compared with the expression level of Nm23-H1 in adjacent tissues(0.84±0.14),the expression level of Nm23-H1 in gastric cancer tissues(0.36±0.11)was significantly decreased(t=2.850,P<0.05).As compared with the control group(0.27±0.08),the expression of Nm23-H1 in gastric cancer tissue was significantly decreased(0.92±0.13)(t=2.189,P<0.05).Conclusion MiR-146a is highly expressed in signet ring cell type gastric cancer,which regulates invasion and metastasis of signet ring cell carcinoma by targeting Nm23-H1.