安罗替尼对胶质瘤放疗的增敏作用及其机制

目的:观察安罗替尼对胶质瘤的放疗增敏作用并探讨其作用机制。方法:(1)培养胶质瘤细胞人脑胶质母细胞瘤系(U87MG)，对照组，置于6MV-X射线照射，安罗替尼组，将安罗替尼加入U87MG联合6MV-X射线照射，细胞克隆法绘制生长曲线，比较两组的放疗敏感性，蛋白质印迹法(Western blot)法检测小窝蛋白(Cave...

Observation and mechanism exploration of the radiotherapy sensitization effect of antinib on glioma

Objective Observation and mechanism of the radiosensitization of anlotinib on glioma.Methods(1)The glioma cell line U87MG was pretreated with anlotinib and irradiated by 6MV-X ray.The growth curve was drawn and the expression of Caveolin-1 protein and phosphorylation level of mitogen-activated protein kinase 1(MAPK)signaling pathway were detected by Western blotting.(2)anlotinib was fed by gavage on the in vivo glioma U87MG model,and the mice were irradiated under 6MV-X ray.Tumor weight was recorded,tumor inhibition rate was calculated,and apoptosis and Caveolin-1 protein expression were detected.Results Cell experiment:sF2,Do and Dq of the control group were 0.64,1.83 Gy and 1.34 Gy,respectively.The values of sF2,Do and Dq in the anlotinib group were 0.47,1.25 Gy and 0.92 Gy.SER value is 1.47.Caveolin-1 protein and MAPK phosphorylation level was decreased.In vivo experiments showed that the tumor volume the anlotinib group and the combined group was smaller than that in the control group,and the tumor weight was(6391.89±114.06),(3367.19±60.61),(4763.02±71.65)and(2982.77±54.30)mg,respectively.Flow cytometry was used to test the apoptosis of tumor cells.Compared with the control group,the apoptosis rates of the radiation group,the anlotinib group and the combined group were increased,and the apoptosis rates of each group were(8.92±0.53)%,(26.75±1.19)%,(24.43±0.79)%and(17.64±0.65)%,respectively.Caveolin-1 expression by immunohistochemical of tumor in mice of radiation group,anlotinib group and combination group was lower than that of control group.Conclusion In vitro and in vivo experiments showed that anlotinib had radiosensitization effect on glioma cells.The decrease of Caveolin-1 expression that modulates of MAPK pathway may be one of the underlying mechanisms of that radiosensitization.