藏红花素对人膀胱移行细胞BIU-87细胞抗增殖作用研究

目的 研究藏红花素(crocin)对人膀胱移行细胞株BIU-87增殖的作用及机制.方法 MTT法测定crocin抗增殖效应,FCM分析细胞周期分布,RT-PCR技术测定Cyclin D1, p27kip1 mRNA表达变化,Western blot检测Cyclin D1,p27kip1蛋白表达变化.结果 crocin对细胞生长有抑制作用,且呈时间-剂量依赖关系.FCM检测显示,crocin可使细胞阻滞于G0/G1期;3.2mmol/L crocin作用于BIU-87细胞后RT-PCR结果显示,Cyclin D1 mRNA表达明显下调,p27 mRNA无显著变化;Western blot结果表明Cyclin D1蛋白明显下调;p27kip1蛋白表达显著增加.结论 crocin对细胞有抗增殖作用,可阻滞细胞于G0/G1期,其可影响Cyclin D1 mRNA转录,但并不影响p27 mRNA表达水平,并调控Cyclin D1、p27kip1蛋白表达.

Inhibitory effects of crocin on human bladder cancer BIU-871000-seed weight cell line

Objective To observe the effects of crocin on human bladder cancer BIU-87 cell line and to explore the anti-cancer mechanism.Methods BIU-87 cells of human bladder carcinoma were cultured by cell culture technique.The MTT assay was used to evaluate the inhibitory effect of crocin for the growth of BIU-87 cells.Flow cytometry was used to measure the cell cycle.RT-PCR and Western-blot were used to detect cyclin D1 and p27kip1 expression.Results The growth of BIU-87 cells was inhibited remarkably in the dosage dependence and time dependence way.Flow cytometry profiles revealed that cells were blocked at G0/G1 phase,the cell proliferation was inhibited obviously.After be treated with 3.2mmol/L crocin,the expression of Cyclin D1 mRNA was down-regulated strikingly,the expression of p27kip1 mRNA was not with significant difference detected by RT-PCR technique.Cyclin D1 protein expression was down-regulated and p27kip1 protin was increased detected by Western blot.Conclusion Crocin can inhibite the proliferation of BIU-87 cells effectivelly,and block cells at G0/G1 phase.The mechanisms may be changing tumor cell cycles by down-regulating the expression of Cyclin D1 gene and protin,up-regulating the expression of p27kip1protin.