| 固相萃取-高效液相色谱法测定枇杷止咳胶囊中吗啡的含量 |
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| 引用本文： | 邱洪,王宝佳,杨立勇,林芳. 固相萃取-高效液相色谱法测定枇杷止咳胶囊中吗啡的含量[J]. 中国药房, 2007, 18(9): 686-687 |
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| 作者姓名： | 邱洪 王宝佳 杨立勇 林芳 |
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| 作者单位： | 1. 解放军第44医院药剂科,贵阳市,550009
2. 贵阳中医学院,贵阳市,550002 |
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| 摘 要： | 目的:建立以固相萃取-高效液相色谱法测定枇杷止咳胶囊中吗啡含量的方法。方法:色谱柱为LichrospherC18(250mm×4.6mm,5μm),流动相为乙腈-0.01mol.L-1磷酸二氢钾溶液-0.005mol.L-1庚烷磺酸钠水溶液(5∶47.5∶47.5),流速为1.0mL.min-1,检测波长为220nm。结果:吗啡进样量在0.2335～1.8680μg范围内呈良好线性关系(r=0.9999),平均回收率为97.6%(RSD=1.2%,n=5)。结论:本方法简便、准确、重现性好,可有效控制该制剂中吗啡的含量。
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| 关 键 词： | 固相萃取-高效液相色谱法 枇杷止咳胶囊 吗啡 含量测定 |
| 文章编号： | 1001-0408(2007)09-0686-02 |
| 收稿时间： | 2006-05-25 |
| 修稿时间： | 2006-10-10 |
Determination of the Content of Morphine in Pipazhike Soft Capsules by SEP-HPLC |
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| QIU Hong,WANG Baojia,YANG Liyong,LIN Fang. Determination of the Content of Morphine in Pipazhike Soft Capsules by SEP-HPLC[J]. China Pharmacy, 2007, 18(9): 686-687 |
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| Authors: | QIU Hong WANG Baojia YANG Liyong LIN Fang |
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| Abstract: | OBJECTIVE:To establish a method for determination of Morphine in Pipazhike soft capsules by using SPE-HPLC.METHODS:SPE condition:C18 cartridge.15mL MeOH-water(3∶ 1)and 5mL water were used as elution solvent.RESULTS:Morphine had a good linearity in the rang of 0.233 5～ 1.868 0μ g(r=0.999 9).The average recovery rate was 97.6%(RSD=1.2%,n=5).CONCLUSION:This method is simple,accurate,and reproducible,and can be used to effectively control the content of Morphine in Pipazhike soft capsules. |
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| Keywords: | SPE-HPLC Pipazhike soft capsules Morphine Content determination |
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