Grapevine virus A: nucleotide sequence, genome organization, and relationship in the Trichovirus genus |
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Authors: | A Minafra P Saldarelli G P Martelli |
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Institution: | (1) Dipartimento di Protezione delle Piante, Università degli Studi e Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy, IT |
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Abstract: | Summary. The 5′ terminal region of the genomic RNA of grapevine virus A (GVA), a tentative member of the Trichovirus genus, encompassing 5 466 nucleotides, was sequenced. Evidence was obtained that the RNA is capped. Two putative open reading
frames (ORF) were identified: ORF 1 that codes for a 194 kDa polypeptide with conserved motifs of replication-related proteins
of positive-strand RNA viruses, and ORF 2 that encodes a 19 kDa polypeptide with no significant homology with protein sequences
from databases. This polypeptide, however, showed 44% similarity with the product expressed by a comparable ORF present in
grapevine virus B (GVB). GVA genome had the same size and structural organization as that of GVB. It also had the same size
of the genome of apple chlorotic leaf spot virus (ACLSV), the type species of the Trichovirus genus, but differed substantially in the number (5 versus 3), size, and order of genes. Differences existed also in the degree
of sequence homology between polymerases, which did not cluster together in phylogenetic trees. Definitive (ACLSV, PVT) and
tentative (GVA, GVB) trichovirus species differ molecularly, biologically and epidemiologically to an extent that warrants
the taxonomic revision of the genus.
Received June 19, 1996 Accepted September 17, 1996 |
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