姜黄素拮抗四氯化碳致肝纤维化作用

目的 观察姜黄素对四氯化碳诱导大鼠肝纤维化的干预作用及机制.方法 32只大鼠随机分为对照组、模型组和水飞蓟宾组、姜黄素组(每组8只),模型组、水飞蓟宾和姜黄素组采用皮下注射体积分数 40%CCl₄复制肝纤维化模型,姜黄素组以姜黄素200 mg/kg、水飞蓟宾组给予水飞蓟宾150 mg/kg灌胃,对照组和模型组以等量生理盐水灌胃,灌胃体积2 mL,连续8周;苏木素-伊红染色和Masson染色观察各组大鼠肝组织病理形态,进行肝纤维化分级;测定肝匀浆羟脯氨酸(Hyp)、超氧化物歧化酶(SOD)和丙二醛(MDA)含量.结果 与模型组比较,姜黄素组大鼠肝组织肝小叶内胶原纤维减少;模型组Hyp、MDA、SOD含量分别为(1.16±0.19)μg/mgprot、(79.50±14.60)U/mgprot、(8.38±0.84)nmol/mgprot,与模型组比较,姜黄素组Hyp和MDA含量[分别为(0.87±0.21)μg/mgprot、(3.95±0.30)nmol/mgprot]降低,SOD活性[(117.74±17.80)U/mgprot)]增加(P <0.01).结论 姜黄素对四氯化碳诱导的肝纤维化具有干预作用,其机制可能与抗氧化能力增强、抑制脂质过氧化反应有关.

Protective effect of curcumin on liver fibrosis induced by carbon tetrachloride in rats

Objective To investigate protective effect of curcumin on experimental liver fibrosis induced by carbon tetrachloride(CCl₄).Methods Totally 32 rats were randomly divided into four groups:normal control group(n=8),model group(n=8),silibinin group(n=8),and curcumin group(n=8).Apart from normal control group,the rats in other three groups were subcutaneously injected 40%CCl4 at the dose of 1.5 mL/kg twice a week for 8 successive weeks to establish liver fibrosis model.Then,the rats in curcumin group were intragastrically administered with 200 mg/kg curcumin;the rats in silibinin group were intragastrically administered with 150 mg/kg silibinin;and the rats in the model group and the control group were treated with physiological saline,respectively,once a day for five days.Liver tissues were stained with hematoxylin-eosin and Massion stain.Pathological changes of liver tissues were examined with micro-scopy and the degree of hepatic fibrosis was assessed with image analyze system.The liver samples were homogenized to examine the level of hydroxyproline(Hyp),superoxide dismutase(SOD)and malondialdehyde(MDA).Results Pathological examination revealed that collagen fibers in liver lobules decreased in the curcumin group compared with that in the model group.Compared to those of the model group,the contents of Hyp(0.87±0.21 vs.1.16±0.19 μg/mgprot)and MDA(3.95±0.30 vs.8.38±0.84 nmol/mgprot)decreased,and the content of SOD( 117.74±17.80 vs.79.50±14.60 U/mgprot)increased in curcumin group(P <0.01 for all)Conclusion Curcumin showed prophylactic effect on CCl₄-induced liver fibrosis in rats and the anti-fibrotic effect may be related to the inhibition of lipid peroxidation and the enhancement of the antioxidant ability.