饮水染镉对大鼠睾丸及血液抗氧化功能影响

目的 探讨饮水染镉对雄性大鼠睾丸及血液抗氧化功能影响.方法 将30只雄性SD大鼠(性成熟)随机分成5组,分别为对照组,25、50、100、200 mg/L CdCl₂组,经饮水染镉,连续56 d;检测大鼠睾丸、血液中谷胱甘肽过氧化物酶(GSH-Px)活性、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量.结果 与对照组比较,200 mg/L染镉组大鼠睾丸中GSH-Px活性[(7.94±2.25)μmol/(min·gprot)]降低(P< 0.05),MDA含量[(5.84±0.61)nmol/mgprot]明显升高(P< 0.01);与对照组比较,200 mg/L染镉组大鼠全血GSH-Px活性[(32.99±4.45)μmol/(min·gprot)]、红细胞SOD活性[(8 574.79±887.65)NU/gHb]、血浆SOD活性[(9.94±1.38)NU/mgprot]明显下降(P< 0.05),血浆MDA含量[(0.26±0.03)nmol/mgprot]明显上升(P< 0.05).结论 饮水染镉可引起雄性大鼠睾丸及血液抗氧化功能损伤.

Effect of chronic cadmium exposure via drinking water on antioxidant function of testis and blood in rats

Objective To investigate the influence of chronic cadmium exposure via drinking water on antioxidant function of testis and blood in rats.Methods Thirty male Sprague-Dawley rats(sexual maturity) were randomly divided into 5 groups and supplied with drinking water containing different concentration(0,25,50,100,and 200 mg/L) of cadmium chloride(Cd) continuously for 56 days,respectively.At the end of the treatment,the samples of abdominal aortic blood and testis of the rats were collected for the detections of activity of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),and malondialdehyde(MDA).Results Compared with the control group,the GSH-Px activity(7.94±2.25 μmol/min·gprot) declined significantly(P <0.05) and the MDA content(5.84±0.61 nmol/mgpr) increased significantly(P <0.01) in testicular tissue for the rats in 200 mg/L Cd group.Compared with the control group,the whole blood GSH-Px activity(32.99±4.45 μmol/min·gprot),erythrocyte SOD activity(8574.79±887.65 NU/gHb),and plasma SOD activity(9.94±1.38 NU/mgprot) declined significantly(P <0.05),but plasma MDA content(0.26±0.03 nmol/mgprot) increased significantly(P <0.05) for the rats in 200 mg/L Cd group. Conclusion Chronic cadmium exposure via drinking water can cause antioxidant function damage in rats.