| 人白介素-2基因在原代培养骨骼肌细胞中的表达研究 |
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| 引用本文： | 潘海燕,田竟生,王珂,赵春礼,郑少鹏,薛绍白.人白介素-2基因在原代培养骨骼肌细胞中的表达研究[J].解剖学报,1999,0(1):52-56. |
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| 作者姓名： | 潘海燕 田竟生 王珂 赵春礼 郑少鹏 薛绍白 |
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| 作者单位： | 1. 首都医科大学神经科学研究所,北京,100054
2. 北京师范大学生物系细胞组 |
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| 摘 要： | 目的 对以人白介素－２在原代培养骨骼肌细胞中的表达作为肿瘤基因治疗模式进行新的探索；方法 将人白介素－２ｃＤＮＡ片段与ＣＭＶ启动子及ＲＳＶ启动子重组，通过Ｌｉｐｏｆｅｃｔｉｎ介导将得到真核表达载体ｐＣＭＶ－ＩＬ２和ｐＲＳＶ－ＩＬ２转入原代培养的骨骼肌细胞，并用免疫组织化学方法观察白介素－２的表达；结果 ｐＣＭＶ－ＩＬ２和ｐＲＳＶ－ＩＬ２经酶切鉴定正确，免疫组织化学观察到约有１０％左右细胞表达了白介
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| 关 键 词： | 人白介素-2 骨骼肌细胞 脂质体介导的基因转移 |
THE HUMAN INTERLEUKIN-2 EXPRESSION IN PRIMARY SKELETAL MUSCLE CELLS OF THE RAT |
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| Pan Haiyan,Tian Jingsheng,Wang Ke,Zhao Chunli,Zheng Shaopeng,Xue Shaobai.THE HUMAN INTERLEUKIN-2 EXPRESSION IN PRIMARY SKELETAL MUSCLE CELLS OF THE RAT[J].Acta Anatomica Sinica,1999,0(1):52-56. |
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| Authors: | Pan Haiyan Tian Jingsheng Wang Ke Zhao Chunli Zheng Shaopeng Xue Shaobai |
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| Abstract: | Objective\ To study
the sex chromosome copy number alterations in lung cancer. Methods\ We detected the copy
number of chromosome X and chromosome Y in touch preparations made from 16 fresh lung
cancer specimens with dual color fluorescent in situ hybridization(FISH). Results\ Increased
chromosome X copy number was observed in 75%(12/16)specimens, including 9 males and 3
females. In 12 male lung cancer specimens, 1 specimen had gained of chromosome Y, 5
specimens had lost of chromosome Y. Conclusion\ Gain of chromosome X frequently occurred
in lung cancers. The relationship between gain of chromosome X and lung cancer genesis
needs further investigations. |
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| Keywords: | Lung Cancer Chromosome X Aberration Fluorescent in situ Hybridization(FISH) |
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