间情期与发情期比格犬子宫与卵巢组织形态学的比较

目的比较比格犬的子宫、卵巢在间情期与发情期的组织形态学的差异,为后期研究奠定基础。方法用化学发光法测定了23只经产比格母犬的血清性激素水平,选取经血清学鉴定处于发情期的比格犬2只,间情期的比格犬4只的卵巢和子宫标本,中性多聚甲醛固定,石蜡包埋,常规HE染色镜检,拍照。结果间情期犬子宫及其内膜较薄,间质纤维增生,黄体中以初级卵泡为主,可见1～2个次级卵泡,未见成熟卵泡,卵泡和黄体细胞间纤维较多、血管少。发情期犬子宫及其内膜增厚,内膜腺体腔较大,部分腺体呈分枝状弯曲,腺上皮肿大,胞浆淡染,少数可见核下空泡。卵巢中卵泡数量较多,有初级、次级和1～2个成熟卵泡。黄体细胞数量多,排列规则,境界清楚,间质纤维比较疏松,血管多,未见空泡变性。间情期和发情期犬卵巢中未见明显白体。结论间情期比格犬的性激素维持在一比较低的水平,在发情前期雌激素水平迅速增高,而在排卵后比格犬的孕酮水平较高。随发情周期的变化,卵巢和子宫在形态学上发生相应的变化。

Comparison of the Uterus and Ovarian Morphology between Diestrus and Estrus Beagles

Objective To compare the uterine and ovarian morphologies between diestrus and estrus Beagles for the further study of their reproductive inefficiency.Methods Detected the serum sexual hormone of 23 Beagle bitches,which have been produced once or twice,using chemiluminescence and selected 2 estrus and 4 diestrus Beagles by serological identification for ovarian and uterine specimens,4% neutral paraformaldehyde-fixed,paraffin-embedded,routine HE stained,photographed.Results The uterus and endometrium of diestrous dogs are thin,interstitial fibrosis,corpus luteum are occupied by primary follicles,1-2 visible secondary follicles,no mature follicles.Follicular and luteal cells have more fiber,less vascularized.While the uterus and endometrium of estrus Canines are more thicken,endometrial cavity is larger,part of the gland is branched bend,glandular cells are enlarged,cytoplasm pale dyed,with a small number of visible vacuoles under the nuclear.Ovarian follicles are in large numbers,and are mainly primary,secondary and 1-2 mature follicles.There are numeric luteal cells which arranged regulatory,state clearly,loose interstitial fibrosis,vascular,and no vacuolar degeneration.Diestrus and estrus dogs’ ovarian have no obvious white body.Conclusion Diestrous Beagle sex hormone is maintained at a relatively low level,in proestrus estrogen levels increased rapidly,and after ovulation progesterone levels were higher.Morphological changes of ovary and uterus are adapted to the estrous cycle of bitches.