| 实验感染登革Ⅱ型病毒的2种蚊媒RT-PCR检测defensin A结果分析 |
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| 引用本文： | 刘先凯,赵彤言,朱礼华,董言德,陆宝麟. 实验感染登革Ⅱ型病毒的2种蚊媒RT-PCR检测defensin A结果分析[J]. 寄生虫与医学昆虫学报, 2003, 10(2): 89-92 |
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| 作者姓名： | 刘先凯 赵彤言 朱礼华 董言德 陆宝麟 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,北京,100071 |
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| 基金项目： | 中国人民解放军总后勤部九五医学杰出青年基金课题资助. |
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| 摘 要： | 白纹伊蚊和埃及伊蚊在经口大剂量感染登革Ⅱ型病毒1天、2天、3天和11天后,用RT-PCR方法都未能在其体内检测到defensin A的mRNA的表达,提示登革Ⅱ型病毒感染其有效媒介宿主白纹伊蚊和埃及伊蚊后并不诱导defensin A的表达,或者表达量极低用RT-PCR方法不能检测到.
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| 关 键 词： | 白纹伊蚊 埃及伊蚊 防御素A 逆转录聚合酶链反应 |
| 修稿时间： | 2001-07-02 |
THE RELATIONSHIP OF DEFENSIN A AND AEDES ALBOPICTUS AND AEDES.AEGYPTI BEING INFECTED BY DENGUE 2 VIRUS |
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| Liu Xiankai Zhao Tongyan Zhu Lihua Dong Yande Lu Baolin. THE RELATIONSHIP OF DEFENSIN A AND AEDES ALBOPICTUS AND AEDES.AEGYPTI BEING INFECTED BY DENGUE 2 VIRUS[J]. Acta Parasitologica et Medica Entomologica Sinica, 2003, 10(2): 89-92 |
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| Authors: | Liu Xiankai Zhao Tongyan Zhu Lihua Dong Yande Lu Baolin |
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| Abstract: | Ae.albopictus Guangzhou strain and Ae.aegypti Haikou strain defensin gene mRNA are not detected by RT PCR after they are infected with dengue 2 virus 1,2,3 and 11 day.This suggests that the infection of dengue 2 virus cannot chanllenge the produce of defensin A,or the amount is too little to be detected with RT PCR. |
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| Keywords: | Aedes albopictus Aedes aegypti defensin A RT PCR |
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