| Protective effect and mechanism of KATP channel opener on the sinoatrial node cells of neonatal rat cultured in simulated ischemia-reperfusion＊ |
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| 引用本文： | 宋治远,仝识非,钟理. Protective effect and mechanism of KATP channel opener on the sinoatrial node cells of neonatal rat cultured in simulated ischemia-reperfusion＊[J]. 中国人民解放军军医大学学报, 2003, 18(4): 205-208 |
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| 作者姓名： | 宋治远 仝识非 钟理 |
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| 作者单位： | Department of Cardiology，Southwest Hospital，Third Military Medical University，Chongqing 400038，China，Department of Cardiology，Southwest Hospital，Third Military Medical University，Chongqing 400038，China，Department of Cardiology，Southwest Hospital，Third Military Medical University，Chongqing 400038，China |
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| 基金项目： | Supported by National Natural Science Foundation of China (No. 30070314) |
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| 摘 要： | Objective: To study the effect of KATp channel opener and its possible mechanism on the sinoatrial node cells of neonatal rats which were cultured under simulated ischemia-reperfusion. Methods: Freshly isolated sinoatrial node (SAN) cells of neonatal rats were purified and cultured for 2 d, and then they were randomly divided into the control, simulated ischemia-reperfusion group (I/R group) , group intervened with KATp channel opener pinacidil (P + I/R group), KATP Channel blocking agent 5-HD (5-HD + I/R group) , and group with the 2 agents at same time (5-HD + P + I/R group) . The survival rate of cells was measured by flow cytometry and the content of intracellular calcium in the cells of each group was detected with laser confocal microscopy. Results: ① The survival rate of SAN cells in I/R group [ (51. 79±6. 28)% ] was remarkably significantly lower than in control [ (95. 08±10. 48)% ] (P < 0.001), and very significantly lower than in P + I/R group [ (63. 77±5. 35) % ] (P<0.01), however, those
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| 关 键 词： | 窦房结 缺血再灌注 KATP通道开放剂 钙 新生小鼠 |
Protective effect and mechanism of KATP channel opener on the sinoatrial node cells of neonatal rat cultured in simulated ischemia-reperfusion |
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| SONG Zhi-yuan TONG Shi-fei,ZHONG Li. Protective effect and mechanism of KATP channel opener on the sinoatrial node cells of neonatal rat cultured in simulated ischemia-reperfusion[J]. Journal of Medical Colleges of PLA(China), 2003, 18(4): 205-208 |
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| Authors: | SONG Zhi-yuan TONG Shi-fei ZHONG Li |
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| Affiliation: | SONG Zhi-yuan TONG Shi-fei,ZHONG LiDepartment of Cardiology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China |
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| Abstract: | |
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| Keywords: | sinoatrial node ischemia-reperfusion KATP channel opener intracellular calcium |
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