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Expression of ZNF396 in basal cell carcinoma |
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| Authors: | Juncheng Bai Yusuke Kito Hiroshi Okubo Tomoko Nagayama Tamotsu Takeuchi |
| Affiliation: | 1. Department of Immunopathology, Gifu University Graduate School of Medicine, Yanagido, Gifu, 501-1193, Japan 2. Innovation Satellite Kochi Practical Application Research, Japan Science and Technology Agency, Kami, Kochi, 782-8502, Japan 3. Department of Dermatology, Kochi Medical School, Nankoku, Japan |
| Abstract: | Zfp191 represses differentiation and keeps various cells in the stem/progenitor stage. Here, we report that a Zfp191 homolog protein, ZNF396, is expressed in basal cell carcinoma (BCC) and possibly represses the expression of a Notch system effector molecule, Hes1 (hairy and enhancer of split-1), and prevents BCC cells from undergoing Notch-mediated squamous cell differentiation. ZNF396 immunoreactivity was found in the nucleus of 35 of 38 cutaneous BCC and 4 of 74 squamous cell carcinoma tissue specimens. In non-tumorous epidermal tissues, ZNF396 immunoreactivity was restricted in basal cells. siRNA-mediated silencing of ZNF396 induced the expression of Notch2, Hes1, and involucrin in cultured BCC cells. Finally, we found that siRNA-mediated silencing of ZNF396 gene inhibited the proliferation of TE354.T basal cell carcinoma cells. ZNF396 might repress Notch-Hes1 signaling axis and prevent tumor cells from undergoing squamous differentiation in BCC. |
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