| HPLC-DAD同时测定川芎CO2超临界流体萃取物中阿魏酸、洋川芎内酯A和藁本内酯含量 |
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| 引用本文： | 刘芳,吴萍,王宇红,蔡光先,柳绍龙,沈晓明. HPLC-DAD同时测定川芎CO2超临界流体萃取物中阿魏酸、洋川芎内酯A和藁本内酯含量[J]. 中国实验方剂学杂志, 2014, 20(10): 48-51 |
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| 作者姓名： | 刘芳 吴萍 王宇红 蔡光先 柳绍龙 沈晓明 |
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| 作者单位： | 湖南中医药大学药学院, 长沙 410208;湖南中医药大学药学院, 长沙 410208;湖南中医药大学, 湖南省中药粉体与创新药物省部共建国家重点实验室培育基地, 中药粉体关键技术及装备国家地方联合工程实验室, 长沙 410208;湖南中医药大学, 湖南省中药粉体与创新药物省部共建国家重点实验室培育基地, 中药粉体关键技术及装备国家地方联合工程实验室, 长沙 410208;湖南中医药大学药学院, 长沙 410208;湖南中医药大学药学院, 长沙 410208 |
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| 基金项目： | 国家重点基础研究发展计划项目（2012CB723503）；湖南省教育厅科研项目（13C672；13C683）；湖南省“十二五”中药学重点学科项目（湘教发[2011]76号） |
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| 摘 要： | 目的:建立川芎CO2超临界流体萃取物中药效组分的定性及定量分析方法。方法:采用HPLC-DAD,Elipse XDB-C18色谱柱(4.6 mm×250 mm,5μm),流动相0.3%乙酸水(A)-乙腈(B)梯度洗脱(0~4 min,40%~41.5%B;4~10 min,41.5%~70%B;10~15 min,70%B),阿魏酸、藁本内酯和洋川芎内酯A检测波长分别为325,328,280 nm。结果:川芎CO2超临界流体萃取物中3种药效组分在15 min内能实现分离,阿魏酸、洋川芎内酯A、藁本内酯的线性范围分别为0.024~2.4,0.15~15,0.3~30μg;平均加样回收率分别为95.87%(RSD 0.99%),96.85%(RSD 1.04%),102.2%(RSD 1.29%)。3种药效组分在川芎药材中质量分数分别为0.35%,3.13%,7.17%。结论:该方法快速简单、客观可靠,适用于川芎CO2超临界萃取物的综合质量评价。
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| 关 键 词： | 川芎 CO2超临界流体萃取 阿魏酸 洋川芎内酯A 藁本内酯 |
| 收稿时间： | 2013-09-03 |
HPLC-DAD Simultaneous Determination of Ferulic Acid,Senkyunolide A and Ligustilide in CO2 Supercritical Fluid Extract of Chuanxiong Rhizoma |
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| LIU Fang,WU Ping,WANG Yu-hong,CAI Guang-xian,LIU Shao-long and SHEN Xiao-ming. HPLC-DAD Simultaneous Determination of Ferulic Acid,Senkyunolide A and Ligustilide in CO2 Supercritical Fluid Extract of Chuanxiong Rhizoma[J]. China Journal of Experimental Traditional Medical Formulae, 2014, 20(10): 48-51 |
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| Authors: | LIU Fang WU Ping WANG Yu-hong CAI Guang-xian LIU Shao-long SHEN Xiao-ming |
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| Affiliation: | School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;Hunan University of Chinese Medicine, Hunan Breeding Base of Key Laboratory Between Ministry and Province for Chinese Materia Medica Powder and Innovative Drugs, Engineering Laboratory Between State and Local of Key Technology of Chinese Materia Medica and Equipment, Changsha 410208, China;Hunan University of Chinese Medicine, Hunan Breeding Base of Key Laboratory Between Ministry and Province for Chinese Materia Medica Powder and Innovative Drugs, Engineering Laboratory Between State and Local of Key Technology of Chinese Materia Medica and Equipment, Changsha 410208, China;School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China |
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| Abstract: | |
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| Keywords: | Chuanxiong Rhizoma CO2 supercritical fluid extraction ferulic acid senkyunolide A ligustilide |
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