Survivin、p53、Bcl-2蛋白在非小细胞肺癌中的表达及意义

目的:探讨Survivin基因在非小细胞肺癌(NSCLC)中的表达,以及与P53,Bcl-2蛋白表达的相互关系。方法:应用免疫组织化学法(SP)检测80例NSCLC肿瘤组织、20例肺良性病变组织中Survivin蛋白、P53蛋白、Bcl-2蛋白表达情况,并将结果进行了相关分析。结果:Survivin蛋白在肺良性病变组织中不表达,在61.3%(49/80)的NSCLC组织中有表达,且Survivin的表达与肺癌患者的TNM分期有关,但与肺癌的细胞类型、分化程度及淋巴结转移无明显关系;肺癌组织p53蛋白阳性表达率55.0%(44/80),与肺癌的TNM分期及淋巴结转移有关,肺良性病变组织中无p53蛋白表达;肺癌组织Bcl-2蛋白阳性表达率50.0%(40/80)明显高于肺良性病变组织的10.0%(2/20),其中鳞癌组织的表达率62.2%,明显高于腺癌组织的表达率34.3%(P<0.05);肺癌组织中P53,Bcl-2蛋白与Survivin蛋白表达显著相关(P<0.05)。结论:Survivin蛋白在肺癌组织中表达上调,提示该基因对NSCLC的发生发展起重要作用。Survivin基因有望成为肺癌基因治疗的新靶点;Survivin蛋白表达与肺癌的TNM分期密切相关,提示可作为判断病情和评价预后的指标。Sur vivin蛋白的表达与p53蛋白、Bcl-2蛋白的表达均呈正相关,三者在肺癌的发生中可能起协同作用。

Expression of survivin protein and its relationship with the expression of p53, Bcl- 2 proteins in non small cell lung cancer

Objective:To study the expression of survivin and its relationship with the expression of P53, Bcl-2 in non small cell lung cancer (NSCLC). Methods:Expression of the survivin, p53 and Bcl-2 proteins was evaluated by immunohistochemical assay in 80 NSCLC tumor samples, 20 inflammatory lung lesions. Results:Expression of survivin protein was detected in a significantly greater proportion of NSCLC (61.3%) than in inflammatory lung lesions (0.0%) (P<0.05); The expression of survivin correlated with TNM stages, and there was no relationship between survivin expression and histologic type ,tumor differation and lymphnode metastasis . The expression of p53, was significantly higher in lung cancers (55.5%) than in inflammatory lung lesions (0.0%)(P<0.05), and correlated with TNM stages and lymphnode metastasis. The expression of Bcl-2, was significantly higher in lung cancers (50.0%) than in inflammatory lung lesions (10.0%), and was significantly higher in squamous cancers (62.2%) than in adenocarcinomas (34.3%)(P<0.05). The expression of survivin correlated with p53, Bcl-2 expression. Conclusion: The upregulation expression of survivin in NSCLC suggested that survivin may play a role in the pathway of carcinogenesis and may be identified as a potential therapeutic target in NSCLC. Expression of survivin correlated with TNM stages, and might be used to evaluate prognosis. The close relationships between the expression of survivin and p53, Bcl-2 indicate that they might play synergetic roles in the process of carcinogenesis of NSCLC.