白血病抑制因子mRNA在早孕期小鼠子宫内膜的表达规律和组织学定位

目的研究白血病抑制因子(LIF)mRNA在早孕期小鼠子宫内膜的表达规律及LIF蛋白的组织定位。方法用RT-PCR半定量法测定LIFmRNA的表达；用免疫组化ABC法测定LIF蛋白的组织学定位。结果LIFmRNA在早孕期小鼠子宫内膜呈时序性规律表达，孕早期升高，第4天达到高峰，比未孕期明显增高(P<0.01)，此后下降，到孕第7天降到孕前水平；LIF蛋白主要定位在子宫内膜腺上皮和腔上皮，且第4天染色最深，间质细胞无LIF表达。结论LIF的表达高峰与小鼠孕卵着床时间吻合。因此，LIF可能参与了着床的启动，LIF参与着床可能是通过自分泌和旁分泌作用的结果。

Expression of Leukemia Inhibitory Factor mRNA and Its Localization in the Endometrium of Early Pregnant Mouse

Purpose To investigate the expression of leukemia inhibitory factor(LIF)mRNA and the location of LIF protein in the endometrium of early pregnant mouse. Methods The LIFmRNA expression was measured by RT?PCR,and the location of LIF protein was determined by immunohistochemistry(ABC method). Results The mRNA expression of LIF was increased during the period of early pregnancy,and went up to the highest level on day 4, then down to pre pregnant level on day 7.LIF was mainly located in luminal and glandular epithelium of endometrium,and the maximal staining of LIF on day 4.No staining was observed in the stromal cells. Conclusions The highest level of LIF on day 4 was coincided with the time of mouse blastocyst implantation.The result documented that LIF might be involved in onset of implantation.LIF may be involveed in embryo implantation via its paracrine/autocrine.