| 紫苏叶中紫苏醛的提取与含量测定 |
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| 引用本文： | 耿榕徽,杨宇杰,胡春兰,杨冬丽,王春民,隋岩.紫苏叶中紫苏醛的提取与含量测定[J].中国当代医药,2014,21(7):4-7. |
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| 作者姓名： | 耿榕徽 杨宇杰 胡春兰 杨冬丽 王春民 隋岩 |
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| 作者单位： | 耿榕徽 (承德医学院中药研究所,承德067000颈复康药业集团有限公司,河北承德067000)； 杨宇杰 (承德医学院中药研究所,承德067000河北省中药研究与开发重点实验室,河北承德067000)； 胡春兰 (承德医学院中药研究所,承德067000颈复康药业集团有限公司,河北承德067000)； 杨冬丽(颈复康药业集团有限公司,河北承德067000河北省中药新辅料工程技术研究中心,河北承德067000)；王春民(颈复康药业集团有限公司,河北承德067000河北省中药新辅料工程技术研究中心,河北承德067000)； 隋岩 (颈复康药业集团有限公司,河北承德,067000)； |
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| 基金项目： | 河南省自主创新重大成果转化项目(项目编号：11276409Z) |
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| 摘 要： | 目的探讨建立紫苏叶中紫苏醛的提取和含量测定方法。方法以紫苏醛为指标，利用气相色谱法（GC）测定其含量。采用DM—FFAP色谱柱，初始柱温为120℃，以2％／min升温至140℃，然后以15℃／min升温至220℃，保持5min。比较索氏提取、加热回流、超声提取法的差异，并优选最佳提取方法。结果建立了紫苏醛的含量测定方法。紫苏醛浓度在19．26～192．63μg／ml范围内线性关系良好，平均回收率为101．68％，RSD=I．97％（n=6）。确定了最佳提取工艺，加入20倍量的无水乙醇溶液，在85℃水浴中回流提取1h。结论本提取及含量测定方法快速简便、结果可靠，可为紫苏叶质量控制提供科学依据。
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| 关 键 词： | 加热回流 紫苏叶 紫苏醛 气相色谱法 |
Extraction and content determination of perillaldehyde fromfolium Perillae |
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| Authors: | GENG Rong-hui YANG Yu-jie HU Chun-lan YANG Dong-li WANG Chun-min SUI Yan |
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| Institution: | 1.Institute of Traditional Chinese Medicine,Chengde Medical University,Chengde 067000,China;2.Jingfukang Pharma- ceutical Group,Chengde City in Hebei Province,Chengde 067000,China;3.Hebei Provincial Key Laboratory of Tradi- tional Chinese Medicine Research and Development,Chengde City in Hebei Province,Chengde 067000,China;4.The New Excipients of Traditional Chinese Medicine Engineering Research Center of Hebei,Chengde City in Hebei Province,Chengde 067000,China |
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| Abstract: | Objective To explore the develop methods for extraction and content determination of perillaldehyde from folium Perillae. Methods Gas chromatography （GC） was applied to determine the content of perillaldehyde.DM-FFAP chromatographic column was applied,with initial temperature set at 120℃ then heated up to 140℃ at the speed of 2℃/min then heated up to 220℃ at the speed of 15℃/min,then maintaining the temperature at 220℃ for 5 minutes.The optimal extraction method was selected based on comparison of Soxhlet extraction,heat reflux extraction and ultrasonic extrac- tion. Results The study developed the method for perillaldehyde content determination.Perillaldehyde had good linear relation at the concentration of 19.26-192.63 μg/ml,with average recovery rate of 101.68%,RSD=1.97% （n=6）.The study identified the optimal extraction process：adding 20 times amount of anhydrous ethanol solution then heat reflux extrac- tion under 85℃ water bath for 1 hour. Conclusion The extraction and content determination methods developed in the study are quick,simply,convenient and reliable,providing scientific basis for quality control offolium Perillae. |
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| Keywords: | Heat reflux Folium perillae Perillaldehyde Gas chromatography |
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