Comparison of SV 40 RNA from infected cells and SV 40 cRNA made by RNA polymerase from Eseheriehia coli

Summary SV 40 RNA from infected AGMK cells and SV 40 cRNA made by RNA polymerase fromE. coli on supercoiled SV 40 DNA are transcribed both largely asymmetrically but mainly from opposite strands. This was found by comparing both RNAs through competition hybridization and annealing experiments. 60–70% of the SV 40 RNA in the cell is transcribed from the opposite strand and 30–40% from the same strand as the SV 40 cRNA made by RNA polymerase fromE coli.