实验高压电烧伤家兔肠黏膜的微循环变化及意义

目的通过观察高压电烧伤后家兔肠黏膜绒毛的微循环变化,探讨高压电对家兔肠道微循环的影响。方法将60只家兔随机分为正常对照组,电击即刻组,电击后1、2、4及8h组,每组各10只。采用实验高压电击设备复制家兔高压电烧伤模型,应用WX-9B型微循环显微镜及视频图像分析系统,观察肠黏膜绒毛微血管形态、微血流动态及微血管周围状态的变化。结果与正常对照组比较,电烧伤各组肠黏膜微血管形态、流态及管周状态均发生变化。电烧伤后微静脉收缩,伤后2h最明显,之后有所恢复,但血流速度减慢;致伤后绒毛血管距绒毛顶端的距离增宽(渗出),以伤后4h最明显;电烧伤后各组均见白微栓,呈逐渐增多趋势;致伤2h可见绒毛出血,对照组与电烧伤后各组比较均有显著性差异(P<0.05)。结论高压电烧伤导致家兔肠黏膜微循环障碍。

Dynamic Alteration and Significance of Intestinal Mucosa Microcirculation After Experimental High-voltage Electrical Contact Injury

Objective By observing the changes in intestinal mucosa villus microcirculation(IMVM) of rabbits after high-voltage electrical contact injury(HECI) to study the regularity and the pathophysiological mechanism of IMVM.Methods 60 adult rabbits were randomly divided into six groups(control group,right after HECI group,1,2,4,8 hours after HECI groups),each contains 10 rabbits.Then imitated the model of HECI,studied IMVM by using the microcirculation microscope(WX-9B) with image analysis system,observed the capillary morphology,the fluid state of capillary and the circumference condition of capillary.Results Compared with the control group,the alteration of IMVM was apparent after HECI,which includes the changes of the capillary morphologry,the capillary fluid state and the capilary circumference condition.IMVM of the HECI rabbits was as follows:the contracted diameter of micro-vein,most obvious alteration 2 hours after HECI,then the micro-vein began to dilate,but the flow rate of blood became slow;the enlarged distance between the villus capillary and the top of villus,most obvious alteration 4 hours after HECI;the white microthrombi formed and grew in quantity little by little;2 hours after HECI,hemorrhage of villus capillary was found;in contrast with the control group,the alteration of IMVM after HECI was significant(P<0.05).Conclusion HECI can lead to the microcirculation disorder of IMVM.