| SD大鼠胰腺导管上皮细胞培养技术的研究 |
| |
| 引用本文： | 龙爱梅 李富荣 李路 齐晖. SD大鼠胰腺导管上皮细胞培养技术的研究[J]. 江西医学院学报, 2005, 45(6): 40-42,53 |
| |
| 作者姓名： | 龙爱梅 李富荣 李路 齐晖 |
| |
| 作者单位： | [1]深圳市人民医院内分泌科,广东深圳518020 [2]深圳市人民医院临床研究中心,广东深圳518020 |
| |
| 基金项目： | 广东省自然科学基金重点资助项目（No.037415） |
| |
| 摘 要： | 目的 分离纯化SD大鼠胰腺导管上皮细胞，为将其转分化为胰岛细胞提供细胞来源。方法 用酶消化法培养SD大鼠胰腺导管上皮细胞，观察细胞增殖动态，用CK-19进行免疫组化鉴定。结果 胰腺导管上皮细胞可在体外有效扩增，有效去除成纤维细胞是扩增的关键。结论 低血清培养和反复贴壁法可获得较纯的胰腺导管上皮细胞。
|
| 关 键 词： | 胰腺导管上皮细胞 培养技术 糖尿病 胰岛细胞移植 动物 实验 大鼠 |
| 文章编号： | 1000-2294（2005）06-0040-03 |
| 收稿时间： | 2005-09-09 |
| 修稿时间： | 2005-09-09 |
The Research of Pancreatic Ductal Epithelial Cells''''s Culture Technology |
| |
| LONG Ai-mei, LI Fu-rong, LI Lu ,QI Hui. The Research of Pancreatic Ductal Epithelial Cells''''s Culture Technology[J]. Acta Academiae Medicinae Jiangxi, 2005, 45(6): 40-42,53 |
| |
| Authors: | LONG Ai-mei LI Fu-rong LI Lu QI Hui |
| |
| Abstract: | Objective To isolate and purify pancreatic ductal epithelial cells for further differentiation towards pancreatic islet cells. Methods The neonate rat pancreas were digested with collagenase and pancreatic ductal epithelial cells were obtained. The cells were observed for their proliferation and were identified with CK 19. Results The pancreatic ductal epithelial cells proliferated better if the fibroblasts were cleared efficiently. Conclusion Low concentration of serum cultivation and adherence repeatedly could let us obtain purified pancreatic ductal epithelial cells. |
| |
| Keywords: | pancreatic ductal epithelial cells culture technology diabetes islet cells transplantation animals, laboratory rats |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
