姜黄素与STI571联合及序贯给药对K562细胞增殖的影响

目的 研究姜黄素(Cur)与STI571联合应用对慢性粒细胞白血病(CML)细胞株K562增殖的影响,探索两药序贯给药的不同效果. 方法 锥虫蓝排染计数计算K562细胞增殖抑制率;显微镜下观察Cur与STI571联合应用对K562细胞的细胞毒作用;锥虫蓝排染法检测药物序贯给药的不同效果;金氏公式评价两药的协同效果. 结果 Cur与STI571单独作用均抑制K562细胞的增殖,二者联合应用呈协同作用(Q＞1.15);显微镜下可见0.4 μmol/L STI571与 5 μmol/L Cur联合应用对K562细胞毒作用强于单独应用;先给予STI571作用24 h,再给予Cur作用24 h,对K562细胞的抑制呈协同作用(Q＞1.15),而先给予Cur作用24 h,再给予STI571作用24 h,则呈单独相加或拮抗作用(Q＜1.15). 结论 Cur与不同浓度的STI571(0.1～0.4 μmol/L)联合应用可协同抑制K562细胞增殖;联合应用STI571和Cur时,先给予STI571再给予Cur比先给予Cur再给予STI571协同效果好.

Combination and Sequential Administration of Curcumin and STI571 on the Growth of K562 Cells

Objective To investigate the effects of curcumin(Cur) alone or combined with STI571 on growth of chronic myelogenous leukemia(CML) cell line K562 and the different effects of the sequential administrations. Methods Trypan blue was used to assess the inhibition of K562 cells and the effects of the sequential administrations. The cytotoxicity of Cur and/or STI571 was observed under microscope. Jin's formula was used to assess the synergistic effects between Cur and STI571. Results Cur or STI 571 inhibited K562 cells with dose-dependent manners, respectively. The combination of the both had synergistic effects on the inhibition of K562 cells(Q >1.15). The sequential administrations showed that synergistic effects were observed when STI571 was first administered for 24 h while Cur was administered for another 24 h(Q>1.15). However, simple addictions or antagonistic effects were observed when Cur was first administered for 24 h while STI571 was administered for another 24 h(Q<1.15). Conclusion The combination of Cur and STI571 shows that they can synergistically inhibit K562 cell growth and the effects of sequential administration of STI571 then Cur is better than first use of Cur then STI571.