抗可提取性核抗原抗体谱检测方法的对比研究

目的比较三种检测抗可提取性核抗原(ENA)抗体谱试剂的敏感性、特异性和检测结果一致性。方法分别采用免疫印迹法(WB)法万孚试剂及纯化抗原的免疫斑点法Trinity试剂和欧蒙试剂检测42例患者(22例SLE患者、10例RA患者和10例非自身免疫病患者)抗ENA抗体谱中的6种抗体(抗Sm抗体、抗RNP抗体、抗SSa抗体、抗SSb抗体、抗Scl-70抗体和抗Jo-1抗体),分析各抗体检测结果的符合率。结果在SLE患者组欧蒙试剂检测抗Sm抗体、抗RNP抗体、抗SSa抗体和抗SSb抗体检测敏感性均高于万孚试剂和Trinity试剂(P<0.05),三种试剂检测结果一致性除抗SSa抗体外均较高。这与检测抗SSa抗体的方法学有关。结论检测ENA抗体谱免疫斑点法优于WB法,三种试剂中以欧蒙试剂检测敏感度较高,且与临床诊断相符。     

Seroprevalence of Anti-Yersinia Antibodies in Healthy Austrians

Yersiniosis is caused by Y. enterocolitica and Y. pseudotuberculosis mostly presenting as intestinal infection. The infection is usually acquired from contaminated food. The aim of this study was to determine the seroprevalence of anti-Yersinia antibodies in Austrians. Sera of 750 healthy Austrians from all nine states were tested for anti-Yersinia IgG antibodies using the recomBlot Yersinia Westernblot kit. Overall seroprevalence was 29.7%. Seroprevalence increased significantly with age from 24.7% in the group of the 19 to 24 year olds to 38.5% in the group of persons older than 44 years. The seroprevalence of anti-Yersinia antibodies varied within the states between 18% and 43.5%. The high seroprevalence of anti-Yersinia antibodies in contrast to only approximately 100 reported yersiniosis cases per year points to the fact that the majority of infections is either subclinical or mild.     

曼氏溶血杆菌Al推定粘附因子基因adh在重组大肠杆菌中的表达

目的　筛选表达曼氏溶血杆菌Al(MannheimiahaemolyticaA1)adh基因的重组大肠杆菌 ,为进一步研究M .haemolyticaA1ADH蛋白及疫苗制备做准备。 方法　IPTG诱导E .coliBRL2 1pAdh4 9表达ADH蛋白并制备多克隆抗体 ,用Westernblot技术分析重组大肠杆菌表达的ADH蛋白 .结果pAdh4 9重组的大肠杆菌能表达分子量约 5 0kDa的蛋白质 ,它能被抗ADH、抗HMW及抗His5识别。结论　pETAdh4 9重组大肠杆菌能表达分子量约 5 0kDa的蛋白质ADH ,它与H .in fluenza的粘附因HMW有共同抗原决定族。提示 :ADH可能是M .haemolyticaA1的粘附因子并能在重组大肠杆菌表达。