Correlations of pseudouridine in 8-hour and 24-hour urinary samples determined by high-performance liquid chromatography

Summary The correlations of Pseudouridine () values in 8 h and 24 h urinary samples from 23 healthy persons were determined. Pseudouridine in the 8 h urinary samples was measured by high-performance liquid chromatography and the 24 h excretion was calculated from the results of three 8 h determinations. Simultaneous determinations of urinary creatinine were performed by Jaffe's reaction. Based on the 8 h values of urinary Pseudouridine the results demonstrated a constant excretion of Pseudouridine in the 24 h periods, if the values were related to the urinary creatinine. The precision in using this ratio as an indicator for normal 24 h excretion of Pseudouridine was 90.5% for males and 92.5% for females.     

糖类抗原CA125水平测定在结核性腹膜炎诊治中的意义

目的了解和评价CA125检测对结核性腹膜炎的诊断和治疗价值。方法采用电化学发光法检测了35例结核性腹膜炎患者和28例恶性腹水患者血清和腹水的CA125水平,并与非活动性肺结核组和正常对照组进行比较。结果结核性腹膜炎组和恶性腹水组血清CA125水平分别为（243.29±131.60）U/mL和（309.05±245.38）U/mL,组间差异无统计学意义（P〉0.05）,但均明显高于正常对照组血清CA125水平（7.13±4.50）U/mL,（P〈0.01）。结核性腹膜炎组和恶性腹水组腹水CA125水平分别为（590.17±275.18）U/mL和（732.39±362.56）U/mL,组间差异无统计学意义（P〉0.05）。结核性腹膜炎组血清和腹水CA125水平均增高者达74.6%,抗结核治疗6个月后,结核性腹膜炎组血清CA125水平下降至（8.47±5.28）U/mL,与对照组相比差异无统计学意义（P〉0.05）。结论CA125水平检测对结核性腹膜炎的诊断价值有限,动态观察CA125水平变化可能有助于预后的判断。     

Time-dependent diagnostic performance of a rapid troponin T version 2 bedside test in patients with acute coronary syndromes

The present investigation confirmed a former report of a diurnal variation with an increased urinary excretion rate of total glycosaminoglycans in the daytime [4]. Fractionation showed that this increase was solely due to three electrophoretic fractions which resisted degradation with chondroitinase ABC. Fasting in the daytime had no influence on the increased excretion of one of these fractions, whereas it prevented the increased excretion of the other two (probably both heparan sulphates). The excretion rates of chondroitin 4/6 sulphate, dermatan sulphate and a fourth chondroitinase ABC resistant glycosaminoglycan (heparan sulphate) were uninfluenced by time of day and fasting. This was contrary to the excretion of hydroxyproline which decreased during the fasting period.     

联合生物标志物检测在慢性肾脏疾病中的临床研究

目的 探讨尿微量白蛋白（mALB）、尿β2-微球蛋白（β2-MG）、尿视黄醇结合蛋白（RBP）、尿转铁蛋白（TRF）、尿半胱氨酸蛋白酶抑制剂C（Cystatin C）及尿N-乙酰-β-D-氨基葡萄糖苷酶（NAG）在慢性肾脏疾病中的临床研究及诊断价值.方法 选择2012年8月～2013年1月在本院住院的慢性肾脏病患者121例为病例组,健康体检者60例为对照组.尿mALB、β2-MG、RBP、TRF及Cystatin C采用酶联免疫吸附法检测,尿NAG采用对硝基苯酚（PNP）比色法检测,并对两组检测结果进行比较及统计学分析,以受试者工作特征（ROC）曲线及曲线下面积（AUC）评价各项标志物单—及联合应用时在慢性肾脏疾病中的诊断价值.结果 病例组中121例患者的尿液生物标志物测定含量明显高于对照组.通过ROC曲线、诊断试验结果显示：病例组中尿mALb、TRF、Cystatin C、NAG、RBP及β2-MG的敏感性显著高于血肌酐（Scr）和血尿素氮（BUN）.尿mALb、TRF、Cystatin C、NAG、RBP及β2-MG的曲线下面积分别为：0.988、0.984、0.974、0.947、0.915、0.897,95％可信区间分别是0.973—1.000、0.963～1.000、0.956 ～0.992、0.910～0.984、0.877～0.954、0.851 ～0.944,联合这6种尿液标志物得到的曲线下面积为0.990,95％可信区间是0.976 ～0.998,差异具有统计学意义（P＜0.001）.结论 病例组中各项尿液标志物的敏感性明显高于Scr和BUN,可以作为慢性肾脏病的诊断标志物.尿nALb、TRF、Cystatin C及NAG的曲线下面积最大,尿RBP和β2-MG也显示了较好的诊断价值.联合应用尿液标志物可以更好地预测临床上急性和慢性肾脏损害的发生,对预防慢性肾脏病的发展具有重要的临床价值.     

多种肿瘤标志物联合检测对肝门胆管癌诊断的意义

目的:探讨联合检测肿瘤标志物CA50,CA125,CA242,CA19-9及CEA对肝门胆管癌诊断的意义.方法:选择近2年间住院且术后病理证实为肝门胆管癌患者90例作为观察组,同期胆道良性病变患者91例作为对照组,采用全自动电化学发光分析仪测定两组患者术前血清中CA50,CA125,CA242,CA19-9及CEA的水平.分别计算两组血清中5种肿瘤标志物的敏感性、特异性及准确性.结果:观察组血清CA50,CA242,CA19-9及CEA的水平明显高于对照组(均P＜0.01),而观察组血清CA125水平与对照组血清CA125水平比较无统计学差异(P＞0.05).血清CA19-9在肝门胆管癌中阳性率最高(86.67％),次为CA242( 63.33％)及CA50 (60％);两组患者血清中5种标志物的阳性率比较,除CA125外,各相应组间差异具有统计学意义(P＜0.05).对于肝门胆管癌的诊断,血清CA19-9灵敏度最好(93.98％),而CEA的特异度最好(94.60％).结论:联合检测CA50,CA242,CA19-9和CEA有助于肝门胆管癌与胆道良性疾病鉴别.     

Does Follow-up of Osteoporotic Women Treated with Antiresorptive Therapies Improve Effectiveness?

The use of biochemical markers of bone turnover has been advocated to improve follow-up of women receiving antiresorptive therapies for osteoporosis, but this strategy is not yet supported by trials showing it improves effectiveness of treatments. To explore the potential value of markers of bone turnover to monitor antiresorptive treatments of osteoporosis, we conducted a decision analysis using a decision tree and Markov modeling. We have compared two strategies: treatment of a woman without specific monitoring; and treatment of this woman with measurement of a serum marker of bone resorption after 3 months of treatment, with change of treatment if response to treatment assessed by this marker was not satisfactory. The base case is the treatment of a 60-year-old osteoporotic woman with a total hip T-score of −3, using a second generation bisphosphonate during 5 years. We found that follow-up produced slightly greater quality adjusted life years (QALYs) than no follow-up (8.1560 vs 8.1532, i.e. a one day difference). In a two-way sensitivity analysis, the follow-up option produced higher QALYs so long as adherence rate with follow-up was equal or superior to the proportion of women who adhered without follow-up. For example, if the proportion of women adherent to treatment was increased from 50% to 60% by follow-up, then the expected value of the follow-up branch was increased from 8.1560 QALYs to 8.1800 QALYs (i.e. a difference of 9 days). In addition, the higher the non-response rate, the greater the benefit from monitoring with a biochemical marker. In conclusion, our decision analysis model suggests that follow-up of osteoporotic women treated with a second generation bisphosphonate during a 5-year period using an early measurement of a serum marker of bone resorption may increase effectiveness of the treatment on quality of life, but the effect is very small. So, the use of follow-up measures of bone turnover may be based on patient and physician preferences. Received: 6 July 2001 / Accepted: 1 April 2002     

Longitudinal Evaluation of Vitamin D Status in Healthy Subjects from Southern Italy: Seasonal and Gender Differences

Vitamin D status is currently considered among the relevant determinants of skeletal integrity. Since vitamin D levels present seasonal variations, we longitudinally studied young healthy men and women in order to investigate the related physiologic modifications of both calcium homeostasis and bone remodeling. Thirty-two men (mean age 39.4 ± 7.8 years) and 58 premenopausal women (aged 36.9 ± 6.4 years) from southern Italy were studied. In all subjects the following parameters were measured both in winter and in summer: serum calcium, phosphorus, creatinine, total alkaline phosphatase activity, 25-hydroxyvitamin D (25OHD), parathyroid hormone (PTH), osteocalcin (BGP), together with urinary calcium (Ca/Cr), total pyridinoline (Pyr/Cr) and deoxypyridinoline (d-Pyr/Cr), corrected for creatinine excretion. In both sexes 25OHD levels were significantly higher in summer, while PTH values were lower, than in winter. The prevalence of hypovitaminosis D, defined by concentrations of 25OHD lower than 30 nmol/l, was 17.8% in winter and 2.2% in summer in the whole sample, while it was 27.8% and 3.4%, respectively, among female subjects. Indeed male subjects did not display hypovitaminosis D, having throughout the year significantly higher calcium and 25OHD levels together with lower PTH values, than the women. Moreover, alkaline phosphatase total activity was more elevated in men both in winter and in summer. In women, during winter, bone remodeling markers levels were higher while urinary calcium levels were lower than in summer. In the whole sample serum 25OHD correlated positively with serum calcium and inversely with PTH. The seasonal percentage variations in PTH were inversely correlated with those of Ca/Cr. Our results show a relatively high prevalence of subclinical vitamin D deficiency among young healthy women from southern Italy. Significant gender-specific differences have been demonstrated in both calcium homeostasis and skeletal remodeling indexes; the seasonal fluctuations in the vitamin D–PTH axis are accompanied by cyclical variations of bone turnover rate, which were more pronounced in women. Received: 11 January 2001 / Accepted: 6 July 2001     

血小板活化因子、癌胚抗原、糖类抗原724在结直肠癌中的表达及与临床病理特征的关系

目的:探讨血小板活化因子(PAF)、癌胚抗原(CEA)、糖类抗原724(CA-724)在结直肠癌(CRC)患者术前血清中的阳性表达率及与临床病理特征的关系、发病的独立危险因素。方法:选取2019年12月至2021年10月于包头医学院第一附属医院就诊的CRC患者75例为CRC组,另选取同时期在本院体检的健康志愿者75例为对照组。采用酶联免疫吸附(ELISA)法分别检测CRC患者术前及对照组的晨空腹外周静脉血PAF含量;回顾性分析上述入组患者术前血清CEA、CA-724含量,进行统计分析。结果:(1)CRC组PAF、CEA、CA-724单独检测和联合检测的阳性率分别为25.33%、42.67%、30.67%、74.67%,均高于对照组,且联合检测的阳性率均高于单独检测指标的阳性率,组间差异均具有统计学意义(P<0.05);(2)单因素方差分析结果显示,血清CEA表达水平的影响因素为患者的淋巴结转移(P<0.05);血清CA-724表达水平的影响因素为患者的年龄(P<0.05);(3)二元Logistic回归分析结果显示,PAF表达在临床病理特征之间的差异无统计学意义(P&...     

Morphometric and Histological Study of Breast Lesions with Special Reference to Proliferative Activity and Invasiveness

Breast carcinoma is the most common cause of carcinoma death in women. Sometimes, difficulty arises to differentiate between premalignant lesions and carcinoma by routine histopathology. Our study was done to establish the role of morphometry and immunohistochemistry to solve this problem. In this study, total 60 cases of different breast lesions were included and 10 controls were also included to compare the results with the normal findings. They were studied by hematoxylin and eosin-stained sections for morphometry and routine histological study; as well as by proliferative markers such as proliferating cell nuclear antigen and p53. Invasiveness was studied using immunohistochemical staining with 34 βE12 monoclonal antibody. Statistically significant differences were found in morphometric parameters and in expression of proliferative markers between most of them. Morphometry and immunohistochemistry help in the proper diagnosis of different breast lesions that lie in the gray zone on routine histopathology.