硒酸酯多糖对癌症患者血清MDA含量及CuZn-SOD活性的影响

目的：了解硒酸酯多糖对肿瘤患者体内ＭＤＡ及ＣｕＺｎ－ＳＯＤ活性的影响。方法：对６０例肿瘤病人随机分成３组：不服硒组，服硒４００μｇ／ｄ组及服８００μｇ／ｄ组并与１５名正常人对照比较。结果：癌症患者血清ＭＤＡ明显高于正常人（Ｐ＜０．０１），血清Ｓｅ及ＣｕＺｎ－ＳＯＤ活性明显低于正常人（Ｐ＜０．０１，Ｐ＜０．０５）；服硒酸酯多糖者化疗后血清Ｓｅ较不服者明显升高（Ｐ＜０．０５），ＣｕＺｎ－ＳＯＤ也明显升高（Ｐ＜０．０１），ＭＤＡ含量显著下降（Ｐ＜０．０５）；服４００μｇ／ｄ组与服８００μｇ／ｄ组之间上述各指标均无明显差异。结论：肿瘤病人摄入适量（４００μｇ／ｄ）硒酸酯多糖可能增加体内ＣｕＺｎ－ＳＯＤ活性，减轻了化疗药物对正常细胞的过氧化损伤。     

硒协同中药多糖对白血病细胞株P388体外作用的实验研究

目的:研究香菇多糖协同硒酸酯多糖对白血病细胞的抑制作用,探讨其相关机理。方法:MTT法检测香菇多糖协同硒酸酯多糖对白血病细胞增殖的抑制作用,流式细胞术检测细胞凋亡,免疫细胞化学检测相关基因Fas和FasL表达水平的改变。结果:香菇多糖和硒酸酯多糖可抑制白血病细胞增殖,硒酸酯多糖主要通过上调Fas和FasL促进凋亡发挥肿瘤抑制作用,香菇多糖主要通过上调Fas促进细胞凋亡,FasL表达没有变化。结论:二者存在不同抑癌机理。香菇多糖协同硒酸酯多糖可显著提高白血病抑制效应。     

Kappa－硒化卡拉胶对阿霉素毒性的预防作用

Ｋａｐｐａ－硒化卡拉胶给大鼠ｉｇ５．１５．４５ｍｇ·ｋｇ－１．ｇｄ．共１４次．在１５．４５ｍｇ·ｋｇ－１剂量组能显著提高注射阿霉素（ｉＰ３ｍｇ·ｋｇ－１，隔日１次．共４次）之大鼠全血谷胱甘肽过氧化物酶活性（Ｐ＜０．０５）．在３个剂量组不同程度改善心电图变化。对阿霉素引起的白细胞、血小板的减少也有抑制（Ｐ＜０．０５或Ｐ＜０．０１）．大剂量卡拉胶组．还可改善阿霉素所致心脏与肝脏的形态改变。Ｐ＜０．０１表３各组大鼠血小板计数值（×１０９·Ｌ－１，）各组均与阿霉素组比较．＊Ｐ＜０．０５，Ｐ＜０．０１＊Ｐ＜０．００１２．２，４对心及肝脏病理切片形态观察给药结束后第５周处死动物，取心、肝脏以３．３×１０３μｍｏｌ·Ｌ－１福尔马林固定，石蜡切片（５μｍ厚），ＨＥ染色，光镜检查。结果表明，阿霉素对心脏有明显的毒性作用，表现为心肌明显的空泡变，灶性肌溶解，而加亚硒酸钠和Ｋａｐｐａ－硒化卡拉胶，其变性程度及范围有改善，表现为肌细胞空泡变性及肌溶解的数量有较明显的减少。阿霉素对肝脏的毒性作用，表现为肝细胞明显的空泡变性及少量的点状坏死，如用硒化卡拉胶或亚硒酸钠肝细胞变性范围程度有不同改善，表现为空泡变性范围缩小，点状坏死减少，尤?     

Schedule-Dependent Effects of Kappa-Selenocarrageenan in Combination with Epirubicin on Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) has a relatively higher incidence in many countries of Asia. Globally, HCChas a high fatality rate and short survival. Epirubicin, a doxorubicin analogue, may be administered alone or incombination with other agents to treat primary liver cancer and metastatic diseases. However, the toxic effects ofepirubicin to normal tissues and cells have been one of the major obstacles to successful cancer chemotherapy.Here, we investigated the effects of epirubicin in combination with kappa-selenocarrageenan on mice with H22implanted tumors and HepG-2 cell proliferation, immune organ index, morphology, cell cycle and related proteinexpressions in vivo and in vitro with sequential drug exposure. The inhibitory rate of tumor growth in vivo wascalculated. Drug sensitivity was measured by MTT assay, and the King’s principle was used to evaluate theinteraction of drug combination. Morphological changes were observed by fluorescent microscopy. Cell cyclechanges were analyzed by flow cytometry. Expression of cyclin A, Cdc25A and Cdk2 were detected by Westernblotting. In vivo results demonstrated that the inhibitory rate of EPI combined with KSC was higher than thatof KSC or EPI alone, and the Q value indicated an additive effect. In addition, KSC could significantly raise thethymus and spleen indices of mice with H22 implanted tumors. In the drug sensitivity assay in vitro, exposure toKSC and EPI simultaneously was more effective than exposure sequentially in HepG-2 cells, while exposure toKSC prior to EPI was more effective than exposure to EPI prior to KSC. Q values showed an additive effect inthe simultaneous group and antagonistic effects in the sequential groups. Morphological analysis showed similarresults to the drug sensitivity assay. Cell cycle analysis revealed that exposure to KSC or EPI alone arrested thecells in S phase in HepG-2 cells, exposure to KSC and EPI simultaneously caused accumulation in the S phase,an effect caused by either KSC or EPI. Expression of cyclin A, Cdc25A and Cdk2 protein was down-regulatedfollowing exposure to KSC and EPI alone or in combination, exposure to KSC and EPI simultaneously resultingin the lowest values. Taken together, our findings suggest that KSC in combination with EPI might have potentialas a new therapeutic regimen against HCC.     

Kappa－硒化卡拉胶的亚慢性毒性研究

Ｋａｐｐａ－硒化卡拉胶是一种新型的有机硒化合物，对其进行亚慢性毒性研究。结果发现血清硒的浓度随Ｋａｋｐｐａ－硒化卡拉胶的摄入量增加而增加；４２０ｐｐｍ浓度可以影响大鼠的生长并诱发肝硬化。     

Kappa-硒化卡拉胶对老年大鼠心、肝、肾、血清脂质过氧化水平的影响

本文通过给予老年大鼠72mg/kg和12mg/kg两种剂量Kappa-硒化卡拉胶(k-SeC)灌胃四个月以观察其对老年大鼠心、肝、肾及血清脂质过氧化产物MDA含量的影响及心、肝、肾的病理改变。结果发现72mg/kg k-SeC组的大鼠心、肝、肾及血清脂质过氧化产物除雄性的肝脏和雌性的肾脏外均有不同程度的增高,但无统计学意义。12mg/kg k-SeC组的老年大鼠其心脏MDA含量明显高于对照组而肝、肾及血清均明显低于对照组,且除雄性肝脏外均有统计意义。病理切片表明各实验鼠脏器均有不同程度的充血、浊肿和空泡样变性,且以高剂量组为明显;苏丹黑染色胞浆颗粒以72mg/kg组稍多。上述结果表明大剂量k-SeC可能会对机体产生毒性作用,而小剂量k-SeC除心脏外能降低脂质过氧化。