S100A4蛋白和上皮钙黏蛋白在大肠癌中的表达及意义

 目的　研究S100A4蛋白和上皮钙黏蛋白（E-cad）在大肠癌组织中的表达及其临床意义。方法　应用免疫组织化学SP法检测87例大肠癌组织及癌旁大肠组织中S100A4蛋白和E-cad的表达水平，分析S100A4和E-cad表达与大肠癌临床病理因素及术后5年生存率的关系。结果　在癌旁大肠组织腺上皮中S100A4蛋白不表达；在大肠癌组织中S100A4蛋白的表达率为64.4 %（56／87），与癌旁大肠组织比较，差异有统计学意义（P＜0.01）；S100A4蛋白在大肠癌组织中的表达与临床分期、淋巴结转移及5年生存率有关（P＜0.05），与其他临床病理因素无关（P＞0.05）。在癌旁大肠组织中E-cad 100 %表达；在大肠癌组织中E-cad的表达率为62.1 %（54／87）；与癌旁大肠组织比较，差异有统计学意义（P＜0.01）；E-cad在大肠癌组织中的表达与临床分期、淋巴结转移、肿块位置及5年生存率有关（P＜0.05），与其他临床病理因素无关（P＞0.05）。S100A4蛋白和E-cad在大肠癌组织中的表达呈负相关，但差异无统计学意义（r＝-0.087，P＞0.05）。结论　S100A4蛋白和E-cad与大肠癌的侵袭、转移及预后密切相关；S100A4蛋白可作为判定大肠癌临床病理特征及判断预后的重要指标。     

大肠癌中β-catenin和E-cadherin的表达及其临床意义

目的探讨β-catenin和E-cadherin在大肠癌发生、发展中的作用。方法应用免疫组织化学方法检测30例正常大肠粘膜、30例大肠腺瘤、10例大肠腺瘤恶变及50例大肠癌组织中β-catenin和E-cadherin蛋白的表达情况。结果大肠癌、大肠腺瘤恶变和大肠腺瘤β-catenin胞浆和(或)胞核异位表达率显著高于正常大肠粘膜(P均<0.01),大肠癌β-catenin异位表达率显著高于大肠腺瘤(P<0.01)。大肠癌中β-catenin、E-cadherin膜表达缺失率显著高于大肠腺瘤和正常大肠粘膜(P均<0.01),且与大肠癌组织分化程度、浸润深度、淋巴结转移、Dukes分期有关。结论β-catenin异位表达与大肠癌的发生有关,是大肠癌发生的早期事件;β-catenin、E-cadherin膜表达缺失与大肠癌的侵袭、转移有关。     

Expression of E-cadherin and uPA and their association with the prognosis of pancreatic cancer

OBJECTIVE: E-cadherin (ECD) and urokinase plasminogen activator (uPA) have been noted as markers for tumor metastasis and prognosis in several tumors. We thus investigated the relationship between the expression of ECD and uPA and the clinicopathological characteristics in pancreatic cancer. METHODS: The expression of ECD and uPA was evaluated in pancreatic cancer tissues from 53 patients. RESULTS: Among 53 tumor tissues, those from 29 (54.7%) patients showed positive ECD expression and those from 22 (41.5%) patients showed positive expression of uPA. There were four subgroups of ECD/uPA expression: ECD-positive/uPA-negative, ECD-negative/uPA-negative, ECD-positive/uPA-positive and ECD-negative/uPA-positive. These patterns were found in 14 (26.4%), 11 (20.8%), nine (17%) and 19 (35.8%) patients, respectively. The tumor tissues with ECD-negative and uPA-positive expression were associated with larger tumor, distant metastasis and an increased clinical stage. There was a difference in the median survival time between the patients with ECD-positive/uPA-negative pancreatic tissues (median: 18.7 months) and the patients with ECD-negative/uPA-positive pancreatic tissues (median: 7.5 months, P < 0.05), and there was a statistically significant difference in survival curves between these two groups. CONCLUSION: The combined analysis concerning uPA and E-cadherin expression may be a useful predictor of metastasis in pancreatic cancer.     

可溶性E-CD在膀胱移行细胞癌患者尿中的含量及其临床意义

目的：检测可溶性上皮性钙粘附素（sE-CD）在膀胱移行细胞癌患者尿中的含量及其临床意义。方法：用ELISA方法测定31例膀胱移行细胞癌患者、17例泌尿系良性病变患者及11例正常人尿中sE—CD的水平．对比各组间指标并结合肿瘤生物学性质进行相关性分析。结果：膀胱移行细胞癌患者尿中sE—CD水平高于正常人和泌尿系良性病变患者：尿中sE-CD水平与膀胱移行细胞癌的分期分级呈正相关．与肿瘤大小及个数无关：复发患者尿中sE—CD水平高于初发患者。结论：膀胱移行细胞癌患者尿中sE—CD水平升高．与膀胱肿瘤的生物学性质有密切关系,对于早期诊断膀胱移行细胞癌以及估计肿瘤的生物学性质具有重要价值     

The Prognostic Importance of E-cadherin and p53 Gene Expression in Transitional Bladder Carcinoma Patients

Objective: The clinical course of transitional cell carcinoma is highly variable. The determination of sensitive prognostic factors for transitional cell carcinoma is very important. Therefore e-cadherin and p53 immunohistochemical activity can be used with other prognostic factors. Methods: The study comprised with 61 (4 women and 57 men) selected patients who had transitional cell carcinoma. Paraffin embedded tissue sections were investigated immunohistochemically for e-cadherin normal staining and p53 over expression. Results: It is seen that when grade and stages of illness increased normal staining of e-cadherin decreased and p53 over expressed. Abnormal e-cadherin was significantly associated with disease recurrence (P < 0.001), disease progression (P < 0.001) and bladder specific survival. p53 differentiation was not significant for disease recurrence (P > 0.05) inverse to prognosis of illness. Transurethral resectomy and BCG treatments were not effected e-cadherin and p53 activity within the groups statistically. Conclusion: Significant differences can be helpful to investigate patients more detailed pathologically. These expression rates in different type of transitional cell carcinoma patients may represent a biologically more aggressive cancer, requiring early definitive therapy. This hypothesis should be evaluated in larger studies and prospective clinical trials.     

转化生长因子β1和E-钙黏附分子在人牙胚发育过程中的表达及意义

目的：观察转化生长因子β1（TGF-β1）和E-钙黏附分子（E-cadherin）在人牙胚发育过程中的表达,探讨其对牙齿发育的作用及意义。方法：胎龄8~34周胎儿尸体36例,取连牙胚的下颌骨,制成4 μm厚的连续石蜡切片。采用SABC免疫组化方法,观察TGF-β1 和E-cadherin在人牙胚中的分布。结果：TGF-β1和E-cadherin在人牙胚发育的不同时期均有表达。TGF-β1在蕾状期和帽状期的牙蕾上皮、牙板、内釉细胞和外釉细胞均呈阳性表达,钟状期在内釉细胞、釉结、中间层、牙板、成釉细胞、成牙本质细胞呈强阳性表达,靠近成牙本质细胞的牙乳头细胞和牙囊呈阳性表达。E-cadherin在蕾状期和帽状期的牙蕾上皮、牙板上皮、钟状早期的成釉细胞、外釉细胞中呈阳性表达,在钟状中期成釉细胞、成牙本质细胞中呈强阳性表达,外釉上皮和靠近成牙本质细胞的牙乳头细胞中呈阳性表达,在钟状晚期接近硬组织处的成釉细胞、成牙本质细胞、上皮根鞘呈强阳性表达。结论：TGF-β1是参与牙胚细胞分化及形态发生的重要调节因子,E-cadherin与牙釉质和牙本质的发育、分泌及矿化密切相关。     

家族性胃癌遗传学研究进展

遗传性弥漫型胃癌与钙黏蛋白基因的种系突变有关,约占家族性胃癌病例的1/3。其他2/3不符合遗传性弥漫型胃癌诊断标准的家族性胃癌依然缺乏明确的分子诊断依据。目前根据钙黏蛋白基因预测情况预防性切除相关家族性胃癌,只在一部分遗传性弥漫型胃癌中有临床意义。     

大肠癌CD44V6、E-cadherin表达及临床意义

目的 :探讨大肠癌CD4 4 V6,E cadherin的表达对大肠癌的进展和转移的意义。方法 :应用SP免疫组化技术检测 56例大肠癌标本的CD4 4 V6,E cadherin的表达。结果 :CD4 4 V6表达有转移者 84 9%( 2 8/ 33) ,无转移者 39 1% ( 9/ 2 3)。E cadherin表达有转移者 2 4 2 % ( 8/ 2 3) ,无转移者 52 2 % ( 12 / 2 3) ,两者与肿瘤转移明显相关 (P <0 0 5)。结论 :对大肠癌组织进行CD4 4 V6,E cadherin的表达检测 ,有助于预测大肠癌的进展程度和转移的判断 ,以及评估预后     

E-钙粘素和β-连环素在妊娠滋养细胞疾病中的表达与意义

目的探讨E钙粘素和β连环素表达与妊娠滋养细胞疾病(GTD)恶性转化的相关性。方法应用逆转录聚合酶链反应技术检测12例正常早孕绒毛和35例妊娠滋养细胞疾病组织中E钙粘素mRNA和β连环素mRNA的表达。结果E钙粘素和β连环素mRNA在正常早孕绒毛与葡萄胎组织的表达差异无显著性(P>0.05);而在正常早孕绒毛、葡萄胎组织的表达量明显高于侵蚀性葡萄胎、绒癌组织(P<0.05);在侵蚀性葡萄胎组织的表达高于绒癌组织(P<0.05)。结论E钙粘素和β连环素的表达下调与妊娠滋养细胞疾病的恶性转化有关,对预测妊娠滋养细胞疾病的恶变以及评价预后有一定的参考价值。     

喉癌中上皮型钙黏蛋白和β-连环素的表达及其意义

目的:探讨上皮钙黏蛋白和β-连环素在喉癌组织中的表达及其临床意义.方法:应用免疫组织化学PicTureTM二步法,检测上皮钙黏蛋白和β-连环素在喉癌组织和癌周正常喉组织中的表达.结果:60例喉癌患者中,上皮钙黏蛋白和β-连环素的异常表达率为55.00%(33/60)和66.67%(40/60),按喉癌的临床分期、颈部淋巴结转移情况分组比较,均差异有统计学意义(均P＜0.05),喉癌的组织学分级比较,差异有统计学意义(P＜0.01),喉癌的原发部位及患者的性别、年龄差异无统计学意义(P＞0.05).同一标本中上皮钙黏蛋白和β-连环素表达有显著的相关性和一致性(P＜0.01).结论:上皮钙黏蛋白和β-连环素的异常表达与喉癌分化、分期、转移密切相关.